FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002862010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002862010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21776099
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1031240.47356507701402345Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG992840.4559310646043628Illumina Paired End PCR Primer 2 (100% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG310310.14250027059483886Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)