FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002863297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002863297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113106520
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT50826034.493642806798405Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1854800.16398700976742983Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT1395160.12334921099155026Illumina Paired End PCR Primer 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)