FastQCFastQC Report
Sat 11 Mar 2023
EGAF00002863303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002863303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71066444
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1472260.20716669037218186Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG941220.1324422536183181Illumina Paired End PCR Primer 2 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)