FastQCFastQC Report
Wed 15 Feb 2023
EGAF00002863324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002863324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157826307
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4936950.3128090680091754Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1947280.12338120539055633Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG1838830.11650972736756743Illumina Single End PCR Primer 1 (97% over 41bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1800680.11409251310682952Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)