FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002869303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002869303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17323
Sequences flagged as poor quality0
Sequence length154
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCCG1309575.5931420654621No Hit
AGCTGCCTGACCCGCACGGACCGCAGTGGAGGCAGCCGCAAAGACCAAGT3482.008889915141719No Hit
CGCTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCCG2711.564394158055764No Hit
AGCTGCCTGACCCGCACGGTATTGGCAGTGGAGGCAGCCGCAAAGACCAA2481.4316226981469722No Hit
AGCTGCCTGACCCGCACGTTCAGTGGGCTCCAAGGTGCAGTGGAGGCAGC2001.1545344339894938No Hit
AGCTGCCTGACCCGCACGGGGACATCGTCACAACATTTCTGCAGTGGAGG1660.9582635802112799No Hit
AGCTGCCTGACCCGCACGGAGCAGTGGAGGCAGCCGCAAAGACCAAGTCT1150.6638572995439589No Hit
AGCTGCCTGACCCGCACGGACCGCAGTGGAGGCAGCCGCACAGACCAAGT1110.6407666108641691No Hit
AGCTGCCTGACCCGCACGGACCGCAGTGGAGGCAGCCGCCAAGACCAAGT830.47913179010563994No Hit
AGCTGCCTGACCCGCACGCGGAAGTGGGGGAGGGAGGTGGAGGGGGGCGG810.46758644576574493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.42140506840616526No Hit
AGCTGCCTGACCCGCACGGGGAATATAATTTTCTTTGGTGCAGTGGAGGC720.4156323962362178No Hit
AGCTGCCTGACCCGCACGGTCCGTGGGCTGAGGTGACAGGCAGAGCAGTG660.3809963632165329No Hit
AGCTGCCTGACCCGCACGCATCCCCCGATGGTGTGTCGCCGCTGGCGGTC610.3521330023667956No Hit
AGCTGCCTGACCCGCACGGACCGCAGTGGAGGCAGCCGCAAAGACCCAGT530.30595162500721584No Hit
AGCTGCCTGACCCGCACGGGGACCTCGTCACAACATTTCTGCAGTGGAGG470.271315591987531No Hit
AGCTGCCTGACCCGCACGGGGGCTGCAGTGGAGGCAGCCGCAAAGACCAA460.26554291981758354No Hit
AGCTGCCTGACCCGCACGCCCAGGGCCACCTCTCTCCGCCCCCAGGCCCG400.23090688679789872No Hit
AGCTGCCTGACCCGCACGCCTCAGCCCTTGGGTGGTCGATGGGACTGGGC350.20204352594816138No Hit
AGCTGCCTGACCCGCACGGCCACCGGCCCCACCTGCAGCTGCTCCGCGGA350.20204352594816138No Hit
GCTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCCGC350.20204352594816138No Hit
AGCTGCCTGACCCGCACGGTCACGTAATGGCAGGCCCCTGGGGAGCCTTG300.17318016509842404No Hit
AGCTGCCTGACCCGCACGCCCAGGGACCCCTCTCTCCGCCCCCAGGCCCG300.17318016509842404No Hit
AGCTGCCTGACCCGCACGGAGCAGTGGAGGCAGCCGCAACGACCAAGTCT300.17318016509842404No Hit
AGCTGCCTGACCCGCACGCCCAGGGCACCCTCTCTCCGCCCCCAGGCCCG300.17318016509842404No Hit
AGCTGCCTGACCCGCACGCCAAGGGCCCCCTCTCTCCGCCCCCAGGCCCG280.16163482075852914No Hit
AGCTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCAAGGCCCG260.15008947641863418No Hit
AGCTGCCTGACCCGCACGGCCACTCAGTAACTTCCTGAAGGGCAGTGAGA250.14431680424868673No Hit
AGCTGCCTGACCCGCACGGAGCAGTGGAGGCAGCCGCAAAGACCCAGTCT240.13854413207873925No Hit
AGCTGCCTGACCCGCACGCAGTGGAGGCAGCCGCAAAGACCAAGTCTCTG240.13854413207873925No Hit
AGCTGCCTGACCCGCACGGTCTTGGCAGTGGAGGCAGCCGCAAAGACCAA240.13854413207873925No Hit
AGCTGCCTGACCCGCACGCCCACTCTACAGCAGTGGAGGCAGCCGCAAAG210.12122611556889686No Hit
AGCTGCCTGACCCGCACGGACCGCAGTGGAGGCAGCCGCACAGACCCAGT200.11545344339894936No Hit
AGCTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCTG200.11545344339894936No Hit
AGTTGCCTGACCCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCCG180.10390809905905445No Hit
AGCTGCCTGACCCGCACGGCACGAGAACCGCCCATGCCTGGCAGTGGAGG180.10390809905905445No Hit
AGCTGCCTGACCGCACGCCCAGGGCCCCCTCTCTCCGCCCCCAGGCCCGC180.10390809905905445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)