FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002871769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22456944
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5824162.5934784358904754No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1032180.45962620737710347No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC529420.2357489068859948No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC294250.1310285139420573No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATACTGATCTCGTATGCCGTC290480.12934974589596876TruSeq Adapter, Index 27 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACATACTGATCTCGTATGCCGTCTT282840.12594768014739674TruSeq Adapter, Index 27 (96% over 32bp)
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTT254910.11351054711629509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)