FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002871772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11041851
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1665521.5083702904522076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC536650.48601452781784504No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC235190.2129987082781682No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT214590.1943424159590634No Hit
CCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGG186310.16873076805691364No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG169480.15348875836125664No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC168960.15301782282698798No Hit
GGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTG139200.12606581994268895No Hit
CGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTA138680.1255948844084203No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCTCGTATGCCGTCT130670.11834066589016642TruSeq Adapter, Index 8 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTTAAATCTCGTATGCCGT118990.10776272927428562TruSeq Adapter, Index 8 (97% over 34bp)
CGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT116810.10578842261139007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)