FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002871776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15803409
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3924972.483622362744646No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC573060.3626179642632802No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC332290.2102647599641318No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT327640.20732235684085631No Hit
CCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGG319850.20239304064078834No Hit
CTCTCGCTATGTTCATCGGGAGCGNNNNNNNNNNN280670.17760092142144773No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG280130.17725922299422864No Hit
CGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTA242770.15361875402958944No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCACACTATCTCGTATGCCGTCT241150.15259365874793218TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACACTATCTCGTATGCCGT231150.14626591009572681TruSeq Adapter, Index 7 (97% over 34bp)
CGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT205770.13020608401642964No Hit
GGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTG202490.12813058245850628No Hit
GCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTA188090.11901862439933056No Hit
TGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG186960.11830358880163135No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG176030.11138735952477088No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGG163360.10337010198242669No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC161100.10194003078702828No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA159090.10066815330793501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)