FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002871786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10443233
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1255331.2020511272706451No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGGCGAATCTCGTATGCCGT158650.15191655687467664TruSeq Adapter, Index 4 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGGCGAATCTCGTATGCCGTCT146250.14004283922421343TruSeq Adapter, Index 4 (96% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC119960.11486864268948133No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGGCGAATCTCGTATGCCGT109720.10506325004909878TruSeq Adapter, Index 4 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)