FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002871787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9918879
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1791511.8061617648526613No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATGCCGT228580.23044942881146144TruSeq Adapter, Index 5 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATGCCGTCT188280.1898198375038147TruSeq Adapter, Index 5 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATGCCGT157030.15831426111761218TruSeq Adapter, Index 5 (97% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC141120.14227414206786876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)