FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002871788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002871788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14820461
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6813134.597110710658731No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCAACAGATCTCGTATGCCGTCT464030.3131009217594513TruSeq Adapter, Index 23 (96% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC459740.3102062749600029No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAACAGATCTCGTATGCCGT446090.3009960351435762TruSeq Adapter, Index 23 (97% over 34bp)
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT406590.2743436928176526No Hit
CCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGG361500.24391953799547803No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC353770.2387037758137213No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG317380.2141498837316869No Hit
CGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTA277080.18695774713080787No Hit
GGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTG258760.17459645823432887No Hit
CGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT231170.155980303176804No Hit
GCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTA212700.14351780285377086No Hit
TGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG207230.13982695949876323No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAACAGATCTCGTATGCCGT204250.1378162258245543TruSeq Adapter, Index 23 (96% over 33bp)
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG197350.13316050020306386No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGG179610.12119056215592752No Hit
CGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGG174710.11788432222182563No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG161270.10881577840257467No Hit
TCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGA156740.10575919332063961No Hit
TTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCG155650.10502372362101287No Hit
TCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG155140.1046796047707288No Hit
TTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATG153320.10345157279520523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)