FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568807686
Sequences flagged as poor quality0
Sequence length66
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30262630.5320362355300523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC7716550.036.2529612
AAGCAGT7774250.036.068721
CAACGCA7977950.035.26806313
ATCAACG8115300.034.9173611
GTGGTAT8604200.034.5112156
GAGTACA9411100.033.2763220
AGCAGTG8517700.033.255972
GTACATG9216350.033.0096222
TACATGG9223800.032.4428523
GTATCAA8956250.031.8653499
GGTATCA9185200.031.6234368
AGTGGTA9452350.031.4636945
CGCAGAG9873000.031.37930716
AGTACAT10093600.030.83983821
TATCAAC9455750.030.79620410
ACATGGG9364400.030.29501524
TGGTATC9845950.030.1587857
CAGTGGT9877850.030.1104534
AACGCAG9638450.029.61920214
AGAGTAC10680250.029.5635419
Produced by FastQC (version 0.11.5)