FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655234046
Sequences flagged as poor quality0
Sequence length66
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41291040.6301723827091854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC7378400.031.63345512
CAACGCA7574500.031.0571513
GTGGTAT8034600.030.267676
ATCAACG7855200.029.97175211
GAGTACA8454900.029.23421120
TATCAAC8348150.028.18158510
GTACATG8684850.028.04364222
TACATGG8784350.027.55737323
AGTGGTA8897250.027.5237625
AAGCAGT8790900.027.4574261
CGCAGAG9221450.027.34807416
GTATCAA8896150.026.4007539
AGTACAT9329800.026.31104721
GGTATCA9235150.026.1116358
AGCAGTG9437400.025.6327232
AACGCAG9549200.025.11241314
GCAGAGT10004150.025.07951517
ACATGGG9663300.024.82886124
AGAGTAC10110200.024.64037719
TGGTATC10090200.024.2214747
Produced by FastQC (version 0.11.5)