FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501317743
Sequences flagged as poor quality0
Sequence length66
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34040520.6790208500559695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6754650.039.5098721
AGCAGTG7375050.038.910992
GTGGTAT9167600.035.1262446
TCAACGC8289650.034.59559612
CAACGCA8460450.034.31289713
TACATGG10032800.033.5411623
ATCAACG8635800.033.40480811
CAGTGGT9792650.033.379824
GTACATG10462550.032.79928622
GAGTACA10935650.032.61487220
CGCAGAG10899800.031.94440516
GCAGTGG10425550.031.8628433
GGTATCA9856850.031.531518
ACATGGG10687100.031.4534524
TGGTATC10369450.031.1667967
GCAGAGT11032450.031.02546517
AGTACAT11428950.030.83439821
GTATCAA9542900.030.8293299
AGAGTAC12546150.028.6662519
CATGGGG6118750.028.51506425
Produced by FastQC (version 0.11.5)