FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603293690
Sequences flagged as poor quality0
Sequence length66
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35544760.5891783817596369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT10352550.044.2341271
AGCAGTG11576750.039.8807642
GTGGTAT14414550.032.824666
TCAACGC14069700.032.69512
AGTGGTA14684400.032.326895
ATCAACG14385500.032.20528411
CAACGCA14384550.032.1627613
GCAGTGG15105250.031.6506393
CAGTGGT15052700.031.5032564
GGTATCA15340300.030.6007048
CGCAGAG15898450.030.56348816
GTATCAA15301150.030.2528259
TGGTATC15659900.030.2105627
GTACATG16050650.029.5605122
GAGTACA16456300.029.3153420
TATCAAC15949200.029.3102210
AACGCAG15938100.029.24831214
AGTACAT16438050.029.1497521
TACATGG16189050.029.09507423
GCAGAGT16708350.028.94175317
Produced by FastQC (version 0.11.5)