FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642932504
Sequences flagged as poor quality0
Sequence length66
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38518100.5991002128584247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC8447300.040.38518512
ATCAACG8669700.039.6961111
AAGCAGT8748900.039.6841
CAACGCA8648650.039.6071213
GAGTACA9550550.037.17853520
CGCAGAG9546100.036.95531516
GTGGTAT9453950.036.8612256
GTACATG9587800.036.5952222
GGTATCA9476550.036.5773478
TACATGG9792500.035.56170323
GTATCAA9885000.034.949419
TGGTATC9972100.034.8847
AGTACAT10328900.034.31368621
CAGTGGT10169200.034.2353484
ACATGGG10102250.034.1294924
GCAGAGT10498400.033.67518217
AGCAGTG10991800.031.665192
AGTGGTA11200600.031.2512955
AACGCAG11679900.029.92676714
TATCAAC11727300.029.68062810
Produced by FastQC (version 0.11.5)