FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603400159
Sequences flagged as poor quality0
Sequence length66
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38371940.6359285695846162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT13691000.049.306511
AGCAGTG18500200.036.6704562
CAGTGGT18521150.036.4169924
TCAACGC19319350.034.78676212
GTGGTAT19498150.034.6673166
ATCAACG19501750.034.6505911
CAACGCA19552350.034.51532713
AGTGGTA20116000.033.706085
TGGTATC20086250.033.604547
GAGTACA20449150.033.583620
GTACATG20252500.033.46449322
CGCAGAG20641550.033.37911216
GTATCAA20220150.033.3072559
TACATGG20247950.033.26225723
GGTATCA20307500.033.256028
AGTACAT21005000.032.5890521
GCAGAGT21124750.032.50948717
ACATGGG20784800.031.94895424
TATCAAC21526650.031.52392610
AACGCAG22154800.030.7156414
Produced by FastQC (version 0.11.5)