FastQCFastQC Report
Tue 19 Apr 2022
EGAF00002873594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643285060
Sequences flagged as poor quality0
Sequence length66
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57980180.9013139524801027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG8690590.13509702836872972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGA8499230.13212229738399334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT24401500.052.5427251
AGCAGTG27093150.047.216492
CAGTGGT32088800.039.8037034
TCAACGC33258950.038.32605712
GTGGTAT33442850.038.274426
ATCAACG33452150.038.23214311
CAACGCA33496950.038.1384413
AGTGGTA34245100.037.4384465
CGCAGAG34487450.037.42463316
GCAGTGG34357650.037.3932343
TGGTATC34219150.037.346697
GAGTACA34418700.037.30743420
GTACATG34239800.037.20751622
GTATCAA34325700.037.1847659
TACATGG34243200.037.09815623
GGTATCA34489550.037.0844238
GCAGAGT35108800.036.68299517
AGTACAT35017000.036.56906521
ACATGGG34911850.036.00140424
CATGGGG19384250.035.20288525
Produced by FastQC (version 0.11.5)