FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002873610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48969131
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15644123.194690140611235Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3479440.7105374199921988Illumina Single End PCR Primer 1 (100% over 50bp)
TTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA1961720.40060339236977677No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1723010.35185635620121586No Hit
TGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGAC1385070.2828455338527449No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG1242880.2538088740026855No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT1207320.24654715641165859No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1154760.23581386404426902No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT1148580.2345518445079207No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC1123380.22940574542766545No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT1023130.20893366476117375No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG960030.1960479960324393No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGT795460.16244111009443887No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGT775330.15833035713866353No Hit
TTGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT710680.14512816247443722No Hit
AACTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTA646770.13207708341812313No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCG617720.1261447747561622No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT591560.12080263380618292No Hit
ACTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCG578520.11813973174243178No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG557980.1139452525714618No Hit
TGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA535650.10938523699756894No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT513000.10475987413376808No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT509650.10407576969254366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509220.10398795927172978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)