FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002873637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89897982
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT15352781.70780029300324TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA5975000.6646422830714932TruSeq Adapter, Index 15 (97% over 40bp)
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT2752470.30617706190557203No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG2699320.3002648046092959No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC2137490.23776840730418175No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT2008510.2234210329660125No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT1388130.15441169747280867No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG1286960.1431578297274793No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT1218310.13552139579729386No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG1162590.12932325889139537No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1161450.1291964484809014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT1150660.12799619906929613TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG1006940.11200918837088022No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC939270.10448176689883873No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT918190.10213688667672206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)