FastQCFastQC Report
Sat 11 Mar 2023
EGAF00002873652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62559053
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC8243381.3176957777797564Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3211680.5133837304090904Illumina Single End PCR Primer 1 (100% over 50bp)
AAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCT843420.13481981576671245No Hit
GTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCC766680.12255300603735161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)