FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002873655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65244867
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC6599711.0115293820738418TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1352450.20728833733387794TruSeq Adapter, Index 10 (100% over 49bp)
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC700930.10743067343519913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)