FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002873658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39242409
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11731532.989503014455611Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5354991.3645925763629851Illumina Single End PCR Primer 1 (100% over 50bp)
TTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA1477780.3765772891261594No Hit
TGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGAC896540.22846201924045997No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG868140.22122495079239402No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT831670.21193143366912054No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC781710.19920030903301578No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT773990.1972330495816401No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720330.1835590674364563No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG640690.16326469661941498No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT634570.16170515933412752No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT550060.14016978417405518No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505390.12878669094957956No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGT504900.12866182603621504No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGT501230.1277266133177502No Hit
AACTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTA486800.12404946903234204No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCG393460.10026397716817027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)