FastQCFastQC Report
Tue 14 Feb 2023
EGAF00002873666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77484352
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4029900.5200921084040298Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1478390.19079852406844675Illumina Single End PCR Primer 1 (100% over 50bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT971270.12535047076343878No Hit
TTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA943100.12171489799643676No Hit
GTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCC879880.11355583124706263No Hit
GAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTG869580.11222653059033132No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT852280.10999382172028747No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC845560.10912654983550743No Hit
AAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCT840710.10850061700199802No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA785380.10135982036734333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)