FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002873705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61727116
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC11459901.8565422690410485TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG794320.1286825064044787TruSeq Adapter, Index 5 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)