FastQCFastQC Report
Fri 24 Feb 2023
EGAF00002873721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66998424
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1686590.25173577217279025TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1498740.22369779921987418TruSeq Adapter, Index 2 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA739180.11032796831161283No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705480.10529799924845994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)