FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002873756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46776932
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12586482.690745087771041Illumina Single End PCR Primer 1 (100% over 50bp)
TTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGA2597190.5552288038044051No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2453550.5245213602294396No Hit
TGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGAC2245730.4800934785547714No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1941220.4149951518838388Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1429670.3056356923964145No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG1282020.27407098866595186No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT1239350.26494897100134746No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC1137570.24319038281518762No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT1115540.2384807964746384No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT926090.1979800641906143No Hit
TTGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT924730.19768932259174246No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG909540.19444199546904872No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT831700.1778013145453832No Hit
TGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA785580.16794175385422883No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT780820.16692415825817736No Hit
AACTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTA776640.1660305554028212No Hit
ACTAGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGT756960.16182335344267554No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGT747470.15979457566819474No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT689780.14746157358075557No Hit
GTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT676020.14451995269805212No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG628040.134262760114323No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCG576490.1232423708335553No Hit
ACTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCG539650.11536669399352655No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA533480.11404766776923292No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA525060.1122476352232763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524250.1120744729474776No Hit
TTGTGTTTGAGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT509790.10898320565359011No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC493420.10548361743775757No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT480660.10275577714245988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)