FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002873815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78493405
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT19942962.540717911269106TruSeq Adapter, Index 13 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5527380.704184001190928No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA5375170.6847925631459102TruSeq Adapter, Index 13 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2911820.37096364974866364No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT2011460.25625847165121707No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG1982900.25261994940848853No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT1784990.22740636617815219TruSeq Adapter, Index 13 (97% over 40bp)
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC1500410.1911510909738213No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT1419310.18081901275654944No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG1097610.139834677830577No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT1085920.13834538073612682No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT1069010.13619105961832081No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG1013470.12911530592920512No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT797270.10157159063235949No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC789000.10051799893252178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)