FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002873825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78720882
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC23585262.9960614516488775TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG9748151.2383181885589136TruSeq Adapter, Index 12 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3234980.410943058285348No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT2026990.25749076337838794No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG1979360.2514402722266247No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC1531790.1945849641267993No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT1474150.18726289169371857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1392760.17692383070606346No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG1198840.15228996036909243No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT1155830.1468263528856295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC1148130.14584821343846224TruSeq Adapter, Index 12 (98% over 50bp)
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT1105940.14048877145456778No Hit
ACTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGG1049290.13329245980755144No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT830740.10552981355061546No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC801620.10183066800496468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)