FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002873831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002873831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72649104
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA56475467.773731111673449TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT53732617.396183440885933TruSeq Adapter, Index 27 (97% over 44bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1326130.18253907164498545TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1322310.1820132564883388TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1303500.17942409860966765TruSeq Adapter, Index 27 (97% over 41bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1126240.1550246235659011TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATTCCTTTATCTCGTAT1015590.13979387825622736TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACATTCCTTTATCTCGTA1010290.1390643441383668TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA947040.13035811150540824TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTTTTATCTCGTA945600.130159898462065TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCTTTATCTCGTA898550.12368356256671796TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCTTTTATCTCGTAT890490.12257412011578285TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT863390.11884386075842036TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCTTTATCTCGTAT838430.11540816800713743TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACATTCCTTTATCTCGTA816790.11242946643911811TruSeq Adapter, Index 27 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATTCCTTTATCTCGTAT781140.10752231713690508TruSeq Adapter, Index 27 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)