FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002874719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14638
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2011.3731384068861867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1821.2433392539964476No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT430.2937559775925673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330.22544063396638886No Hit
ATTTAGAGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCACTATTGAAATGGCATCAACATG220.15029375597759256No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAA210.1434622216149747No Hit
AGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAA190.12979915288973903No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC190.12979915288973903No Hit
CCTCAACGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA190.12979915288973903Illumina Single End PCR Primer 1 (100% over 58bp)
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT190.12979915288973903No Hit
GTTTATTAATTCAAATAACTTATGTGAGAGCTGCTATAACCATTTCCCCAGTTCAACATTGCATTGAAAAT190.12979915288973903No Hit
TGAGGACACATTTTGGCATTCTTGCTGACTCTGACAGAGTGATTCTGATGGACTTGTATTTAAATTGTATG180.12296761852712118No Hit
CCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTCAATGTGGATGAGAACTAATCGCTGATCGTC180.12296761852712118No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA180.12296761852712118No Hit
CTGTTATAGTCATTTTGTCTTTTAATTTATTAGGACTAAAGATTCATACACCTGCAGTGTGTTATTTGTGG170.11613608416450336No Hit
CATATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAATGTCACCACTATCTGGAGA170.11613608416450336No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC160.1093045498018855No Hit
ATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTT160.1093045498018855No Hit
GTAAACACTCAAAACCAAGCAAAAAACATTTGTAAGATACACGGTATCTATTTGGAGCAACGGTTTTTGTA160.1093045498018855No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1093045498018855No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA150.10247301543926765No Hit
GATAATTGTGATTTTCTCTTTATAACCCAGGAGCTTAAAACTAACACATTACCACATTGTTTCAGCAAAGG150.10247301543926765No Hit
GTATATAGTTGTAGAATACATTATTTGATCTCTAAAACATAGTTCTGAATGCCTTTGATTCACAGAAAGAA150.10247301543926765No Hit
AACTTGATGACAGTAAAACCCTGTTTAGTATTCTCTTGTGCATTTGGTTTGTTGGTTAGCCGACTGTCTTG150.10247301543926765No Hit
GCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTA150.10247301543926765No Hit
CCTCTCCTGTGACTGATAACGTATATGTTATCAGTCACAGGAGAGGAGGGAATGAAGAAGAAGAAATATTA150.10247301543926765No Hit
ATTTTCCACTTAACCAGGAAATATTTCTCCTCTAAATGCATGAAATCATGTTGGAGATCTCTATTGTAATC150.10247301543926765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)