FastQCFastQC Report
Wed 8 May 2024
EGAF00002874757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855426
Total Bases60.7 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68570.801588915931945No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA52030.6082349612941389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33570.39243604940696214No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC17840.20855106110873414No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA15910.1859892030403565No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG13920.16272594005793606No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT10990.1284740000888446No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT10720.1253176779756519No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC10440.12204445504345204No Hit
TTCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGG9650.11280929034188814No Hit
GGGTAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTCCACAT9570.11187408378983102No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA9280.10848396003862402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)