FastQCFastQC Report
Tue 7 May 2024
EGAF00002874772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16209
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.7218212104386451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.715651798383614No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA780.48121414029243015No Hit
TCCCTACACTCCGAAGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG360.22209883398112162Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT330.20359059781602812No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG320.19742118576099696No Hit
CCCTACACTCCGAAGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT270.1665741254858412Illumina Single End PCR Primer 1 (100% over 31bp)
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC270.1665741254858412No Hit
GAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACA250.1542353013757789No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT240.14806588932074774No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC230.14189647726571658No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14189647726571658No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC220.13572706521068542No Hit
TCCGAAGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT220.13572706521068542Illumina Single End PCR Primer 1 (100% over 39bp)
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG200.12338824110062312No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA200.12338824110062312No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC180.11104941699056081No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATC180.11104941699056081No Hit
TAACAGAACTGAGAAACTTTTATATATAACTGATGATCACATAAAACAGA180.11104941699056081No Hit
GTTCCAAAGAGAATGTTTGAAAAAATATTCAACCTCAGTAACATTTGAAT170.10488000493552964No Hit
CCTTAATTCTAATTTTATTTTGTTCTCTACATACAATCAATGTAAAGCCA170.10488000493552964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)