FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002874794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAG714.285714285714285No Hit
GCGTAGAAGTATCAATCAGCCGGTTGCCTTTGTGAGAAGAATTCCTTGGACTGCGGCGTCGAGTCAGCTGA48.16326530612245No Hit
CATCTTTGGAGCTCTTACTGTCCTAAACTGTACAGCTGTGCTTAAAACCCTTATTTCATATAAATGGCCTT48.16326530612245No Hit
GCCTTCTCCCATGGGATGACTCAGCAAGAAGACCCTTGTTAAATGTGTGCCCCTCAGCCTTGGACTTAGCC48.16326530612245No Hit
CCTTTAAATAAAGCGTTTGTGTTTCAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36.122448979591836No Hit
GAGTATCATTAAGAGTAGTGATGGGAAGATTACAGTCTGAGGGCCAAACGTGCCTGCTTCCTGTTTTTGTA36.122448979591836No Hit
GTGTCATAGAATTAATTTTCAGCCATGTGGACCATATTTTGTATCCAAGGATCCTTATTTAAAGCTTTCAA24.081632653061225No Hit
GGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAAGATAGCCCAGAGGAAAAAATAAAGCAATTAGAGAAGGA24.081632653061225No Hit
CTCCGGTTGGCCTATCAGTGACCACCAGAGGACAATGGGAAGGGCCCTTTCCTAAAGACAGAGCACGAAAC24.081632653061225No Hit
GGCCGAAGTGGGAGGATCTCTTGAGCCCAGGAGGTCGAGGCTGCGGTAAGCAGTGATTGTGCCACTGCACC12.0408163265306123No Hit
CTCTCTTCCAGGACATCAAGAAGCCAGCTGATGATGAGTGGGGTAAAGCCCCAGCCGCCATGAAAGCTGCC12.0408163265306123No Hit
GTTTCATGCACATCGTCCTAGAATTAATTACCCTAAAAATCTTTGAAATAGGACACGTAATTACCCTATAT12.0408163265306123No Hit
GAGTATCATTAAGAGTAGAGATGGGAAGATTACAGTCTGAGGGCCAAACGTGCCTGCTTCCTCTTTTTGTA12.0408163265306123No Hit
ATTCATAAGATTTTTTAAATTGTCATAATTATATTTTAAATACCCATCTTCATTAAAGTATATTTAACTCA12.0408163265306123No Hit
GTTTCATTCCCATCGTACTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACACTATAA12.0408163265306123No Hit
GTGTCTATTGAGGTGCTTAGCAATAAAGAAAGGTAGTGAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.0408163265306123No Hit
GATTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAG12.0408163265306123No Hit
ACTAAGACCCTTCTGAGCCCACCGACTTCTGAAGCGCCCCATGCAAACTAATAGTGCTTCTGCCTAAGCCT12.0408163265306123No Hit
GGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.0408163265306123No Hit
GCCTTCTCCCATTGGATGACACAGCAACAAGACCCTTGTTAAATGAGTGCACCTCAGCCTTGGACTAAGCC12.0408163265306123No Hit
GTGTCTATTGAGGTGCTTAGCAATAAAGAAAGCAAGTGAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.0408163265306123No Hit
CTCGATGCTAAGTATCTACATGGATACATTAAATATATTTATGCGAGTTGCAACTATGCTGGCAACTGGAG12.0408163265306123No Hit
GGTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.0408163265306123No Hit
CTAATCCATTTCCCTAACCTAGTAACCTCCAGATCCCAGAGGCTCTCCTCACCTCAGCTGAGCTCCTTTGA12.0408163265306123No Hit
AATAAAACATTGCAAAGTACCCAAGGACCAATTCCAAATTTTCCTTTAAGAACCTTTAATAATATTAAAAA12.0408163265306123No Hit
GACCAATGCTTCACTCAGCCAAATTACAACACCACAAAAAAAAAAAAATAAAAAAATATAAAAAAAAAAAA12.0408163265306123No Hit
GTCAACGATCCCTCCCTTACCATCAAATCAATTGTCCACGAATGGTACTGAACCTACGAGTACTCAGACTA12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)