FastQCFastQC Report
Wed 8 May 2024
EGAF00002874867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194364
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.37198246588874484No Hit
CTACTAAGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT7100.3652939844827231Illumina Single End PCR Primer 1 (100% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4880.2510753020106604No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA4040.20785742215636643No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT4010.2063139264472845No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC3390.17441501512625796No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT3250.16721203515054228No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA2720.13994361095676153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)