FastQCFastQC Report
Wed 8 May 2024
EGAF00002874874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712879
Total Bases50.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145682.0435445566498665No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA27540.38632082022334785No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG17510.24562373137657303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17170.24085433853430946No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA13890.19484372523247284No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC12980.18207858556641449No Hit
TCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT9960.1397151550263088Illumina Single End PCR Primer 1 (100% over 46bp)
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT9220.12933471178138226No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT8590.12049730739718803No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC7490.10506691878986475No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.10282249862879957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)