FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002874949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002874949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931
Sequences flagged as poor quality0
Sequence length71
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29615.32884515794925No Hit
AATGTAATACATTCCAGTCTACACTTTGTTTTTGTATTGTTGCTCCTGAACAACCTGTTTCAAATTGGTTT492.5375453133091663No Hit
GTGTTTTGGTGTTTTGCACTTCTGTAAACTTACTAGCTTTACCTTCTAAAAGTACTGCATTTTTTACTTTT472.4339720352149143No Hit
GTAAAATTCGAGCCATATAAATAAAACCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTT472.4339720352149143No Hit
GTGTGTGGGTTCTGATCCCAGGACTGCTACTGTGGCAGTCATGGCTAGAACTCAAGAAAATGCATCAGAAA452.330398757120663No Hit
CTGGGTCAAGGAGTAAGCAGAGGATAAACAACTGGAAGGAGAGCAAGCACAAAGTCATCATGGCTTCAGCG452.330398757120663No Hit
CATCACAAAAGAGTTGATTGAAATTATCTCTGGTGCTGCAGCTCTGGATTAATGAAAATCAAGTTCCATCC422.1750388399792855No Hit
GTACTGGGATGGGGAGAGGCGGACCTATGTTCCCGCCCTGGAGCAGTCGGCCGACGGACATAAGGAGACAG381.9678922837907822No Hit
ATGTAATACATTCCAGTCTACACTTTGTTTTTGTATTGTTGCTCCTGAACAACCTGTTTCAAATTGGTTTT361.8643190056965304No Hit
TTATCTCTGGTGCTGCAGCTCTGGATTAATGAAAATCAAGTTCCATCCTCAGACAAGAGGTAAAGAAGGAA351.8125323666494046No Hit
CTGGCTGTACTGTTGGCTTTACCTGTTTTTTTTTAACGGTGTGTGGGTTCTGATCCCAGGACTGCTACTGT291.5018125323666496No Hit
GTGTGAAGTCAAATGCATGCCAACATTCCAGTTTTTTAAGAAGGGACAAAAGGTGGGTGAATTTTCTGGAG281.4500258933195236No Hit
GTCTTGGCTGCTTTACGACCCATTTTGAACTTGAATAAGAAAATTGCTTGAATTTGCTTTTTGTCTAACAT251.294665976178146No Hit
GTGTGGTCAAGGACCTTAAAGACAAGGCCAAAGAGACTGCAGATGCCATCACTAAAGAAGCGAAGAAAGCT211.0875194199896427No Hit
GCCCCAGAGCCTGGGAAGGAGGCCGCTATGCAGGGTAGCACTGGGAACAGGAGACCCACCTGAGGCTCAGC201.035732780942517No Hit
GTTCAGTGTTCTGACTCGCCATGGAGCTACACCAGAATCAGGAGCATTTTGTACCCAAGGCCCTTATCACT190.9839461418953911No Hit
GTCCTGAAGAAGTGGTACACGATTTTTAAGGACCACGTGTCTCTGGGGGACTATGAAATCCACGATGGGAT190.9839461418953911No Hit
GGACTTTAATCTTGGTGATGCCTTGGACAGCAGCAACTCCATGCAAACCATCCAAAAGACCACCACCCGCC190.9839461418953911No Hit
GTTTTTATCATTGAAGACAATATTGAACAACCCCCTATTTTGTGGGGATTGAGAAGGGGTGAATAGAGGCT170.8803728638011392No Hit
GTGTGCGTTAGACAAATGAACAGTGTTGTGTACCTGTGTGAATTGTCACTGTGACTCCAGGCTCTAGGCTC150.7767995857068877No Hit
CGATTCACTCAGCTGGCTGCTACTTCGCCTTCCCTGCACCACACTCTGCCAGGTCCTGCATGCTGTGAGCC140.7250129466597618No Hit
GTCTCTGGCATTGTTCCTCATTTATGCCATAGTCAAAGAAAAATATTACCGGCATTTCCTGCAGATCACCC140.7250129466597618No Hit
GTATCAGTCAGTGCTGGAGAAATCTTGAATAGCTTATGTACAAAACTTTTTAAATTTTATATTATTTTGAA120.6214396685655101No Hit
AGTGAAAACTGGTACAGTGTTCTGCTTGATTTACAACATGTAACTTGTGACTGTACAATAAACATAAGCAT120.6214396685655101No Hit
GATGTATAAAGAATGCTATGAATAAAACATTAAGAAGCTGTGTAATTTTAAAAAAAAAAAAAAAAAAAAAA120.6214396685655101No Hit
GGTTTGGGGACATTATAAACTTGAGTACATTTGTTGTACACAGTTGATATTCCAAATTGTATGGATGGGAG110.5696530295183843No Hit
GCTGTACTGTTGGCTTTACCTGTTTTTTTTTAACGGTGTGTGGGTTCTGATCCCAGGACTGCTACTGTGGC100.5178663904712585No Hit
GGGAGATATACCTAATGCTAGATGACACATTAGTGGGTGCAGCGCACCAGCATGGCACATGTATACATATG100.5178663904712585No Hit
GCATCGCCCTAGTGCTCTGCGTGGCGGAGAACGTGTTTGCTGTACGCTGTGCTCAGCTCACCCACCAGCTG100.5178663904712585No Hit
CCTGTCCAGATGATGAGGAGATCGAGCTCGCCTATGAGCAAGTGGCAAAGGCCCTCAAATAAGCCCCTCCT90.4660797514241326No Hit
GTTGATGGCCCCATCTGGATGTAAATCCTGAGCTCAAATCTCTGTTACTCCATTACTGTGATTTCTGGCTG90.4660797514241326No Hit
GTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGTGGAGCA90.4660797514241326No Hit
AGTTCAGATAAGAAAATTAAAGTTACTTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.4660797514241326No Hit
ACGCGGGACAGCGACTGCAGCTGAGTCATGATCTGCTCGGAATCCTCCTGCTAAAGAAGGCTCTGGGCGTG90.4660797514241326No Hit
TGGTGATATAGGGAAAAGAGAACAAATTTGAATTTGTAATAATAAAATGTTTAATTATAGAAAAAAAAAAA80.4142931123770067No Hit
TTACTGCTGCCTTTGTCCGAAGAAACTGTTCCTCCATTATTTGAATTACTGAAGACAGCAAGATATTTGTA80.4142931123770067No Hit
ACAATTACCTAAAACCTCGGGCAGAAGAGGAGAGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAA80.4142931123770067No Hit
GATTAAAATTAATACTAATGACGTAATTAGCCTCAAAGACTTGTACTAATCTGTTATTTTTGGGTCTTCGA80.4142931123770067No Hit
GTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGTGGAGCC80.4142931123770067No Hit
GTCCTCGGCCTAACGAAGGACGTGAACCTTGTAAGAGGAGAGCTCTGAAACGTGGTCACCTTTGTTTAGTG70.3625064733298809No Hit
CATGTACACTTGGCTGTCAGGCTTTCTGTGCATGTACTAAAAAAGGAGAAATTATAATAAATTAGCCGTCT70.3625064733298809No Hit
GAGTGCAAGGAGGAGGACATCATGTGCATGTATGAAGCCGAAATGCAGTGGAAGAGGGACTACAAAGTCGA70.3625064733298809No Hit
AAACAATATGGTTTTAAGGAGTATTACATTAGCACAGCTCTCCTCTCTTTATGGAACCTAAAATGTTGAAC60.31071983428275507No Hit
GCACTCATGATATGGAAAGGCTTGATCGTGCTCACAGGCAGTGAGAGCCCCATCGTGGTGGTGCTGAGTGG60.31071983428275507No Hit
AAAGGGAACAGTGTGGAGATGTTTTTGTCTTGTCCAAATAAAAGATTCACCAGTAAAAAAAAAAAAAAAAA60.31071983428275507No Hit
GAATGGGACCCTGTTGATGGCCCCATCTGGATGTAAATCCTGAGCTCAAATCTCTGTTACTCCATTACTGT60.31071983428275507No Hit
CCTTCATCCTTGACTCCTTTGGTATTTCACTGAATTTCAAACATTTGTCAGAGAAGAAAAAAGTGAGGACT60.31071983428275507No Hit
GCTGAAAAAGAAATCAGCCTATGGTTTAAGCCTGAAGAACTGGTTGACTACAAGTCTTGTGCTCATGACTG60.31071983428275507No Hit
CGCCTATATTAAGGCACATTTGAATAAATTCTATTACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.25893319523562924No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG50.25893319523562924No Hit
GTTTGGGAATTTACATAATATAAAGGAGATGATTACTGATAGTTGCAGTCAATTCAGTATAGACAGTGGAG50.25893319523562924No Hit
AAAAAAATCTTTACATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.25893319523562924No Hit
GCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAGGTGCTTAAGCGGCTGCGACTTCAGA50.25893319523562924No Hit
CCTTCATCCTTGACTCCTTTGGTATTTCACTGAATTTCAAACATTTGTCAGAGAAGAAAAAAGTGAGGACA50.25893319523562924No Hit
CAGTTTCCTTTCTTAGCTGATGTCTTTGGCCAGAACACCGTGGGCTGTTACTTGCTTTGAGTTGGAAGCGG40.20714655618850336No Hit
GCTGTGTGGCTCTGTATGACTGTTGCTGAAATATAAAGCCCTGCAACCTGAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
GACTTGTAGTAACTGCCACCAATAAAGCAGTCTTTACCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
TCCTTAAAGTTGCCATATAGATGTGAACTGCTCAACACACAGATCTCCTACTCCATCCAGTCCTGAGGAGC40.20714655618850336No Hit
ATTTGCAGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
GTTCCAGTTTCTCTCCTACTCCCATCTGGTTGTGCTCGGTGAAGTAGGTGAGCAGTGCTACCCTTCTGAAA40.20714655618850336No Hit
GTGATATAGGGAAAAGAGAACAAATTTGAATTTGTAATAATAAAATGTTTAATTATAGAAAAAAAAAAAAA40.20714655618850336No Hit
CCTGGGTTTGTCCGCATGTTGTAATCGTGCAAATAAACGCTCACTCCGTAAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
CACATGTTGCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
ATTTGAATTACTGAAGACAGCAAGATATTTGTAAATTATCTTAAAATAAACAACTTAAAATAAAAAAAAAA40.20714655618850336No Hit
CAATAGTCTTATTAAAATAAATGTTTTTTAAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20714655618850336No Hit
CCTGTCCAGATGATGAGGAGATCGAGCTCGCCTATGAGCAAGTGGCAAAGGCCCTCAAATAAGCCCCTCCG30.15535991714137753No Hit
TTTCAGTTCCCTCCTGCCCAGGACTGCATTGTTTTCTAGCCCCGAGGTGGTGGCACGGGCCCTCCCTCCCA30.15535991714137753No Hit
CTGCCATTCTGCATGAGCTGATCAGCCTGTGCCACACTTCGCTCTTGAGGAGCTCAACGGTCTGGCAGGGC30.15535991714137753No Hit
GTCCTCGGCCTAACGAAGGACGTGAACCTTGTAAGAGGAGAGCTCTGAAACGTGGTCACCTTTGTTTAGTT30.15535991714137753No Hit
GTTTGTGGCGCCTGTCATGGCAGGCATCTCATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15535991714137753No Hit
TTATTATTATGTCCTACAAGCATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15535991714137753No Hit
AGTGAAAACTGGTACAGTGTTCTGCTTGATTTACAACATGTAACTTGTGACTGTACAATAAACATAAGCAA30.15535991714137753No Hit
GTACAAAACAGGTCTGTTTCAGAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15535991714137753No Hit
GGAGGGCCCTGGGAAGAATTTGCCATTTTCAGTGGAAAACAAGTGGTCGTTACTAGCTAAGATGTGTTTGT30.15535991714137753No Hit
GATGTTATTTAGACAGAAAGAGTGATGAACGGCTAACTGCCTTCCGGAACAAGAGTATGTTATTTAAAAGG30.15535991714137753No Hit
CTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGGAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GGTAAAAAGGGAAGGATGAGGCTCCCCCAATGTCACATTAAATTCATGGTTTTCATTCAAAAAAAAAAAAA20.10357327809425168No Hit
GACTCCGGCAGCTTTATCGCCAGAGTCCCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATCACCG20.10357327809425168No Hit
CAGCCAGGCCAGGGTAGGCAGTGCCTGCTTCTGCTCCATCAGGTGCAGGGGATTTGGCTGAAGGCGTGCAT20.10357327809425168No Hit
ATTAAATGGTTGAGAACAATGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GTATTCTCATGGCCTGATTTTTTTTGTTTCTATTCCTTTTATATCACTGTGTTTGAATCGAGGGGGAGGGG20.10357327809425168No Hit
TTTCAGTTCCCTCCTGCCCAGGACTGCATTGTTTTCTAGCCCCGAGGTGGTGGCACGGGCCCTCCCTCCCC20.10357327809425168No Hit
GGTAATGCTTGTTATCTTCCATCTAATAAAAATCTGCTGCAGATGTGTAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GCCACATGTGCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GTGTATGTACATATATATATTTTTTAATTTGATGAAAGCTGATTACTGTCAATAAACAGCTTCATGCCTTT20.10357327809425168No Hit
GTAAAATTCGAGCCATATAAATAAACCCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTT20.10357327809425168No Hit
CTGGCTGTACTGTTGGCTTTACCTGTTTTTTTTTAACGGTGTGTGGGTTCTGATCCCATGACTGCTACTGT20.10357327809425168No Hit
GGTTTTTATCATTGAAGACAATATTGAACAACCCCCTATTTTGTGGGGATTGAGAAGGGGTGAATAGAGGC20.10357327809425168No Hit
ACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGCCCAGTG20.10357327809425168No Hit
GAATCTCCCTCTTTTTTTTCCCGGATTATTATGTGACTTTAACATTCACTGCAATCCTTCATGGGGTAATA20.10357327809425168No Hit
AGATTTTACCAAATGTTGCAAGAACTCTGGAGTTCTTATGGTAGTAAAATGCCGGAAAGAAAATTCTGCAT20.10357327809425168No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT20.10357327809425168No Hit
TCCCTACACGACGCGGGGGGGGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GGGCAGCACCTAGGATGGGGCAGAGACTTGTCACATCTTTGTCCCCAGCAAAGGCTACATGTTACCTCCTT20.10357327809425168No Hit
GTACTGGGATGGGGAGAGGCGGACCTATGTTCCCGCCCTGGAGCAGTCGGCCGACGGACATAAGGAGCCAG20.10357327809425168No Hit
CAGTAGAGTAGAAGTCTCTTCCAGTTTGCTTTGCCCTTCTTTCTACCCTGCTGGGGAAAGTACAACCTGAA20.10357327809425168No Hit
GCCAAGGACATGGAACGCTGGGCCCGCAGTCTCAACAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG20.10357327809425168No Hit
TTCCCTACACGACGCGGGGTCTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
ATTTAACATGTGGAAGAAAACAAGACATGAAAAGAGTGGTGACAAATCAAGAATAAACACTGGTTGTAGTC20.10357327809425168No Hit
GATGCCCTTGAATTAATAATAAAAAGTTGAAGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)