FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14014
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8035.729984301412873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.4210075638647067No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG450.3211074639646068No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT290.20693592122163548No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTA290.20693592122163548No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGG270.19266447837876408No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC240.17125731411445697No Hit
CCGTATGGCCCGTGCCAACGCCCGGCTCTTCGGCATACGGGCAAAAAGAGCCAAGGAAGCCGCAGAACAGG240.17125731411445697No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAA220.15698587127158556No Hit
ACGCGGGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGC220.15698587127158556No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGA210.14985014985014986No Hit
GTATGTATTCTCTTTTGTGTCATTCTTTCACTCAACATAATTTTGTTGAGAATCATCTATGTCCTAACAGC210.14985014985014986No Hit
AGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTA190.13557870700727845No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG180.12844298558584272No Hit
GGTTTGGGATGGATGCACCTAGGAAAACATGTCCAAGGTTATGGGGGGCGGTGGCTGCACCCTACTGGCAG180.12844298558584272No Hit
GATTATGACCTTGTTTCTTTAGATTTGATTTTCCAAGTTTGAAAAGACCAGTTTAAAAATGACTTTTGCTG180.12844298558584272No Hit
TCCCTACACGACGCGGGAGCCTTTGGGGAAAATTAACTTTTTCTTTTGATAAGTATGATATTCAATAAAAT170.12130726416440703No Hit
CAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAA170.12130726416440703No Hit
GTACTATAGACATAATTCAATAGTCTGTAAACCCTTTGTAAATTACCAGTGGTGAGTCTTTAAACCCAAGA170.12130726416440703No Hit
GAATTACAAAATAGTTTTTAAATATTGTCTGAGAAAAGCCAAAGTTAATGCAACCTAGTGGAAACTGTAAG170.12130726416440703No Hit
AGCCAACATAGCAAAACCTCTTCTCTACTAAAAATACAAAAATTAGCTGGATGTGGTGGCATACACCTGCA160.11417154274297131No Hit
GTCCCTACTAAAATACAAAATTAGCCAGGCATGGTGCCACGTGTCTGTAATCCCAGCTACTCAGGAAGCTG160.11417154274297131No Hit
GAAAGAGACACAGATATTTGTGGAAAAACTTTTTGATGCTGTGAATACAAAGAGTTACCTACCTCCTCCAG160.11417154274297131No Hit
CATTTAGTTTTGTTTCAAAACATTTCTCTTCCTACAGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACTCT160.11417154274297131No Hit
GTCAGGAGGTCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAATTAGC160.11417154274297131No Hit
ACTCTGACCCATAAGGATTTGTGTTGTAAACTGTGGGATAACCATAAAGAGAAAAATGAAAGAATTCATTC150.10703582132153562No Hit
CTTCATGGCTGGGTCCAGCAGAAAAGAGGCTGAATCTTCTCCATTTGTTGAGCGACTTCTGAAAAAGGGCT150.10703582132153562No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGG150.10703582132153562No Hit
TAGGAGGAGAGGGAGGACAAAGGAGAGCCTTTTCTTTTGTCCATTACTCTTAGAAATGAACAGGTACATAA150.10703582132153562No Hit
CTCCGGATCTGCATAGTGACCAAAGAGGGCATCAGGGAGGAAACTGTTTCCTTAAGGAAGGACTGATCTGT150.10703582132153562No Hit
GTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACC150.10703582132153562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)