FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842.664129400570885No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG621.9663812242308911No Hit
CCGCTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA561.7760862670472566Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250.7928956549318109No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT200.6343165239454488No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA150.47573739295908657No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA130.41230574056454167No Hit
GTTTTTGCTTATACACAATTCATTCTTTGCAGCTAATTAAGCCGAAGAAGCCTGGGAATCAAGTTTGAAAC110.3488740881699968No Hit
CCGCTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTTAAAA110.3488740881699968Illumina Single End PCR Primer 1 (100% over 58bp)
CTTCTTAGATTGTTAAATAAAATAAATTATAATAAACTGTTAACTCTTTTCAGTATTTAATACCTGTAGTT100.3171582619727244No Hit
CTTAATTCCTATTTCAATATGTGTTTCCTTGAAGAATTCAGGATACAACTTCTTGTGTATGACAGCTTTCC100.3171582619727244No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC90.285442435775452No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT90.285442435775452No Hit
CTTCTTCGCTATTTAAGTTGGATATTTCTCTACATTCCTGAAACAATTTTTAGGTTTTTTTTGTACTAGAA90.285442435775452No Hit
GTACTGTTTCAAATCAAACCTCAGGGATTTAGAGATACTGTTGTTACTTCTGCATGTTCATTGCAAACAGA90.285442435775452No Hit
TTCTAATTCAGATCATCAAACTGATTATATAGAAGAGTTGGCTTTAAAATGTTTGCAAATGTCTTTTTTTT80.2537266095781795No Hit
GGCCAACCTGGTGAAACCCCATCTCTACTAAAAATACAAAAATTACCCGGGCATGGTGGTGGGCACCTGTA80.2537266095781795No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTT80.2537266095781795No Hit
GGAAAAATATGAACATGATATTTTATAGCTTGGGAAGAAACTTGCAGATGATATGTGCTGCCTTTGCTTCT80.2537266095781795No Hit
GCCATCATTCTGTCTACCTGCAAGGGTCTTTTGTGTAATATTAGTGTCATTTAAATCTGTTTCAGAATAGA70.22201078338090707No Hit
CCTGAGACAGGAGTTGGAGGAGTAGAGGGTGGCGCTTGTTAAGTGAAACATTTAACCAGGAATGGTATTGT70.22201078338090707No Hit
GGTGCAGTGGTGCATATCCGTAATCCCAGCTACTTAGGAGGTTGAAGCAGAATTATTGCTTGAGCCCAGGA70.22201078338090707No Hit
GTTTTGTGTGGGATAATTTGAAGAATATATTATGAGTAAACTCCGAAAATTTTGTTTATCCAAAGGCTCAA60.19029495718363465No Hit
GCAGGAGATGCAGACTCAGGAGTCAATGCAGAAGTATCCTATTCATTTTTTGATGCTTCTGAAGATATTTT60.19029495718363465No Hit
GTATCATCACCATTGGTTTCATAATGTAAATACTATATGTTGAACAAATTAAATGTCAAAATTTTTTATTA60.19029495718363465No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG60.19029495718363465No Hit
AGGTAGATAAAGAGGATGAAATTACTGAAAAGAAAGCCAAAAAAGCCAAGATTAAAGTTAAAGTTGAAGAA60.19029495718363465No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAA60.19029495718363465No Hit
GCTGTAAATAAGGTCATTAAGTCTTATGCCAAATTCAGTGCTACTCCTCGTTACATGACATACAACTGAAC60.19029495718363465No Hit
GTGTAGAAGCATTCCTTCTTTGATAATGTTAAATTTGTAAGTTTCAGGTGACATGTGAAACCTTTTTTAAG60.19029495718363465No Hit
CTGTCCACCAAGATCAAGCAGAGAAAATAATTAATTTCATGGGACTAAATGAACTAATGAGGATAATATTT60.19029495718363465No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCA50.1585791309863622No Hit
GGATGTTCCTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAA50.1585791309863622No Hit
TTGAAGTTGGTGGTGGTCGGAAAGCTATCATAATCTTTGTTCCCGTTCCTCAACTGAAATCTTTCCAGAAA50.1585791309863622No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG50.1585791309863622No Hit
GAAACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAGGGGGCCTTCC50.1585791309863622No Hit
GCTCTCCTTGTCCCAGCTTTGTGATTGAGCCCTCTGTCTTGCTCTCTCTGCCTTCCCTGTGTCTTTCTTCT50.1585791309863622No Hit
ACAATATTTTCCAGTGTTAAAAAGGAAGGCAAACCTTCTGAGACAAAATGAAGGGCAGAGGAAACCAGTAG50.1585791309863622No Hit
GTACATTACCATATAATGTAAAAAGATCTACATACAAACAATGCAACCAACTATCCAAGTGTTATACCAAC50.1585791309863622No Hit
GAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAAGATAAATTTAAACCTG50.1585791309863622No Hit
ACAATCAGGCTCACTGCAGCCTCTACCTCCCAGGCTCAAACAGTTCTCCCACCTCAGCCTCCTTGAGTAGC50.1585791309863622No Hit
GCTATACGAGAGAATCCAGTCTATGAAAAGAAGCCCAAGAAAGAAGTTAAAAAGAAGAGGTGGAACCGTCC50.1585791309863622No Hit
CCTCTGGGTGATCCAAGTGTAGTGGGACCCCCTACTAGGGTTAGGAAGTGGACACTAACATCTGTGCAGGT50.1585791309863622No Hit
CATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGAT50.1585791309863622No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA50.1585791309863622No Hit
GAAGGAAGAGCCTCAAAATAAGTGTGCACCCATGCACATATTCAGGAACAGCTTGTTTAGTCTTTACACTT50.1585791309863622No Hit
CTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGAAA50.1585791309863622No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA50.1585791309863622No Hit
GAAACAACTATTTTAAAAGAACTCTTTTAAGAAATTTCATAAAGGGACATGCATTTTACTGGGTTTGCTTT50.1585791309863622No Hit
CTGCTTGTCATCCACACAACACCAGGACTTAAGACAAATGGGACTGATGTCATCTTGAGCTCTTCATTTAT50.1585791309863622No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC50.1585791309863622No Hit
ATCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTTTGTTCA50.1585791309863622No Hit
TCCCTACACGACGCGGGGTTCAGCAGGCTGATGAATAAGATCTAAGAGTTACCTGGCTACAGAAAGAAGAT50.1585791309863622No Hit
GCCGAAGATACTATCAGTATTCTCCCTGATGACCCCCGACAAATGACTCTCTTATTCTAAGGATCCTTCTA50.1585791309863622No Hit
CATAAAAAGAGGAGGCAATCGCTTTGAGCCATATGCCAATCCAACTAAAAGATACAGAGCCTTCATTACAA50.1585791309863622No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAC40.12686330478908975No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC40.12686330478908975No Hit
CATTTATCTGTATATTATGTTTACTTCTAAGGATATAGCACAAAGGGAACATTTTTTGTTTAAAGTGAACT40.12686330478908975No Hit
ACACCTGTAGTGTAAAGTGGCCACTATGAATCCCTATGTATGAGAGGAGGGAGGCAGGCTGCAGCTTCAGC40.12686330478908975No Hit
GCTTTTCACGTGATTAAGACCCTACTCCAAATTGTAGAAGCTTTTCAGGAACCATATTACTCTCATGATAC40.12686330478908975No Hit
GTATAATGAGAGCAGGGCTATTGTAGTTCCCAGATTCAATCCACCGAAGTGTTCACTGTCATCTGTTAGGG40.12686330478908975No Hit
CTTTTTATGTTAAAAAATGTACATTTACTGTGGATTCTGTTTAATTTATTGTGTATGTGTGGGGAAAAGAT40.12686330478908975No Hit
GTAGCTCAGGAGAGCACCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGT40.12686330478908975No Hit
AATCATGGCTCACTGCAGCCTCTACCTCCCGGGCTGTAGCCATTTTCCCACCTCAGCCTCCCAAGTAGCTA40.12686330478908975No Hit
CCCTCAGTTGAGCCTGAGCCCCCGGCGCGGGGGCTGCTACACTACAGGACACCCAGCATCGGCTTTGACTG40.12686330478908975No Hit
CAATGTGGCTTGCTCCTTTTTGAATCTACAGATAATGCATGTTTTACAGTACTCCAGATGTCTACACTCAA40.12686330478908975No Hit
GGTCCCAGCACCGATGGCATTCCCTTGCCCTGAGTCTGCAGCGGGTCCCTTTTGTGCTTCCTTCCCCTCAG40.12686330478908975No Hit
GAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAA40.12686330478908975No Hit
GTGCTGATAGAAATGTACTTGCCAAGCAGGCTGTTTCTTGTCCCCAGAAAGCTGAAATTTACATAATATAC40.12686330478908975No Hit
AGACAGACTGGCTTCTAGCCACCTTAGTTTTCCTTGGCAATGTTTCCTAGTCTTGATACTTGAAGAGCAAG40.12686330478908975No Hit
ATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACACAGAGGAGAGAAGTTAAACAGGGTAAAGAT40.12686330478908975No Hit
GGCATTCTGAGGGGCAACGTGGAGGAAGGGCCAGGGATGCATGGGATTTTAATTGTTTCATCACACCTTCC40.12686330478908975No Hit
GGTTCGTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12686330478908975No Hit
GGTGCGGTGGCGCACTCCAGCTTTGGGCAATAGAGCAAGTCTCTGTCTCGAAATGAAATGAAGTAAATGAA40.12686330478908975No Hit
GCCTATAACCATCTTTTTGAAACTAAGCGTTTTAAGGGTACTGAAAGTATAAGTAAAGTGTCTGAGCAAGT40.12686330478908975No Hit
GTGCCTCACAAAGGCCACCGAATTCTTAATCTCTTTCAGGTTTACTCAACTAATAAGTAACTTGTAGAGTT40.12686330478908975No Hit
GGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATACTTCACAACAATCCT40.12686330478908975No Hit
GGCCACGGCCTCCTCCTTTGGGGAGGTCCTTAGGATCTCAGCTTGGCAGTCGAGTGGGTGGCGACCTTTTA40.12686330478908975No Hit
GACCAAAGCGATTAGGAGATACTAAAAACAGGGGAAACCAGACAGGAGAATATTACACTAGAGCAAGCACA40.12686330478908975No Hit
GGCAACATAGTGAGACCTTGTCTTTATAGAAAATTAAATTAGCCAGGTGTGGTGGCATGTGCCTATAGTCC40.12686330478908975No Hit
GTTCTCAGAGGCATGGGTGGTGCTTTTGTGCTTGTCTTGTATGATGAAATCAAGAAGTACACATAAGTTAT40.12686330478908975No Hit
GGGAATCTATGGCATAGACGTCTAGATAGCAGTTAGTTTAAGAAGAGTTTCTAGAGGTGAGGAGTATTGAG40.12686330478908975No Hit
ATAATGACTTCAGCAAATAAAGCAATCGAATTACAACTACAAGTGAAACAAAATGCAGAAGAATTACAAGA40.12686330478908975No Hit
GATAGAAGATGCTGTTCTTCTGAGAGATACGTTACTCTCTCCTTGGAATCTGTGGATTTGAAGATGGCTCC40.12686330478908975No Hit
CTACTTGGCCAGTCTGTAGGCCAACAAGAAGGTTCCTTTACCCCCATGCAAGACACTTATGAGAACACATT40.12686330478908975No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAATCAAACCACTTTCACCGCTACACG40.12686330478908975No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT40.12686330478908975No Hit
GAGTTTATTGTATTCATTGGAATATGTGACGTAAGCAATAAAGGGAATGTTAGACGAGAAAAAAAAAAAAA40.12686330478908975No Hit
GGTTGAGCTAGAGATACAAGTTGGGAGTTCAAAAGATCGTATTTGAAACAATGGAAATACTGAGATGATTC40.12686330478908975No Hit
CCCCCCCAATCGGACTGCCAAATTCTCCGGTTTGCCCCGGGATATTATAGAAAATTATTTGTATGAATAAT40.12686330478908975No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCAT40.12686330478908975No Hit
AAGTTAAATAGTTGACAAAGTATGTATTTGCTGGTGTCGTGTAAATATTGGTATTTTAAAATAAAAACTGT40.12686330478908975No Hit
CCTGTGTTCCTGGTTATCCTCTTCTGCTGTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12686330478908975No Hit
ACCTCTCTTTGCCTGTAGGATGTACTGTATGTAGTCATGCACTTTGTATTAATATATTAGAAATCTACAGA40.12686330478908975No Hit
ATAGTAAGTTGGGCAAGGTAGACATAATGAATAGTTGCAATATACCTTAAGAGAGATAGGAATAGAGGCTT40.12686330478908975No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC40.12686330478908975No Hit
CAAAACACACCATGCCAATAAATGAGATGAAAACTTGAGTTTGCCTTTTTAACTATTTATGTTCTAAGTTA40.12686330478908975No Hit
GTCTGTTACCCCTCGCAGACTCCGTAACCAAACTCTTCGGACTGTGAGCCCTGATGCCTTTTTGCCAGCCA40.12686330478908975No Hit
GTACAAGATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGTCCTAG40.12686330478908975No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTAT40.12686330478908975No Hit
GGCCGCACTCTTTCTGACTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGG40.12686330478908975No Hit
ATGTACACTGTTGAGTTTTCTGTACATAAAAATAATTGAAATAATACAAATTTTCCTTCAAAAAAAAAAAA40.12686330478908975No Hit
CATCTTCATGTGGGCTGGGGTAGAAACTGGTGTCTGCTCTGGTGTGATCTGAAAAGGCGTCTTCACTGCTT40.12686330478908975No Hit
GGTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATATACAC40.12686330478908975No Hit
GCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGGTTCGAGGGCACGAGT40.12686330478908975No Hit

[WARN]Adapter Content

Adapter graph