FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46021.56586966713549No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG120.5625879043600562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.5157055789967182No Hit
TAGGGGATGTCAGTTTCCTATGGAAGAGACACCTCTGACCCGTTATTCTTATAATCAAAATCTGAAGGGAA110.5157055789967182No Hit
TGATAGTTCTGCTGATGTTTTGTTTATTGGGCAGACATATCTTCATTAAGAGTTTTTGGAAAACTCATCAA80.3750586029067042No Hit
GCTCTGAGGATAAAGCAAGTTCTAATTTGTGGAAAAAGGATTAAATATTTCTGTTTTCTGAAAAGAGTTAT70.32817627754336615No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATC70.32817627754336615No Hit
CAATCTTCGTGATTGGCTTCGAGTGGTTGCATCTGACAAGGAGACCTACGAACTTCGTTACTTCCAGATTA70.32817627754336615No Hit
GTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCACCCTGCCTGCACCTCCGCGGGCTGACTGGGCCACAG70.32817627754336615No Hit
GTTTTGTGGACCACTTTAGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTAC70.32817627754336615No Hit
TTCATAAACATTTTTTATTTGTATAATTGCAACAGTTTAAGAAATTATCACAACTATTTAGAAACATTTAA60.2812939521800281No Hit
GTCTATGAGTTTTCAATTCATTTCGGTTTCATGTACAGTATTACATTTATGCAACCAAGCTATCAAAAGGA60.2812939521800281No Hit
ATCTTTACCTCCTTGTCCCAAACCATCATTATATGTGTCCTGTACTCTTGAAATAGCCTACTAACTGGTGG60.2812939521800281No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT60.2812939521800281No Hit
GAGTTGGGAGGATGGCTTGAGCCTGGAGGTTAAGGCTGCACTAAGATCACGCCACTGCACTCCAGCCTGGG60.2812939521800281No Hit
CCCCCAGGTGGAGCAACATGCGATTCTGGAGGCACGGGGGTAACTGAAAGTGAGTACATATAGTCTTTCTG60.2812939521800281No Hit
GAGAATGGCATGAACTTGGGAGGCGGAGCTTGCAGTGAGCCAAGGTCGCGCCACTGCACTCCAGCCTGGCC60.2812939521800281No Hit
AATTGATCTGTCACACTCAGGCTCATGGGCAGGCACAAGAAGGGGAATAAAGGGGCTGTGTGAAGGCACTG60.2812939521800281No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC60.2812939521800281No Hit
CATTTTTTCCAGAGTGGTGCAGTACCACTGAGGACTGTTGCTGTATTGATTAGGAAAAGAGACAGAGTAAT50.23441162681669012No Hit
GTGCTGCCCCCCTCTTAATACTCACCAATAAATTCTACTTCCTGTCAAAAAAAAAAAAAAAAAAAAAAAAA50.23441162681669012No Hit
CATTTCCAAACCATCAGCTGCTTTTAAAATAAGATCTCTTTGTAGCCATCCTGTTAAATTTGTAAACAATC50.23441162681669012No Hit
GTGTGAGAGAAGGGTTAAGACTGGCTTACAATGAGATGCTTCAAATGAAAAGGGAATTATGAGTAAAATTG50.23441162681669012No Hit
GAATGATTTCTGTACCTAAAGACATTTAAAAATTTGTGCAATAAAAGATAAACCATAGACTTGGAGTCAGT50.23441162681669012No Hit
GCCTTCTGTACTTGAAGTTGATTTGAAATATGTAAGAATTGATGATGTATTTGCAAACATTAATGTGAAAT50.23441162681669012No Hit
ATCTAATACTATCTTACGTCTAATTAGTGTAGCATTTACAAGAAAGAAAAATTAAGATCTTAAAATCAGTG50.23441162681669012No Hit
GATTAGAACAAAAACATAGCATAGAGAATATATAGGTTTTAATGATTACATTAGAAAATTAGGCTGGAAAT50.23441162681669012No Hit
GAACTTGAAGTCCTAATTAAATGTTCTTCAGGTAGAAGACCTAATATTGTTGAAATGGGCTGGGTGTGATG40.1875293014533521No Hit
AGTTAGCTACGGGATTCTGTTTGACTCGATGCGAGCAAACCATGGTTACTCCAACACGAAACTGACATGAA40.1875293014533521No Hit
AGGCATATGGGATTTATACAGATTCACTTGTAAATGTTGGATTGGGGATTTTTGTGTAAATTTTCTCAAAT40.1875293014533521No Hit
AATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTAACCTGAATCGGAGGACAACCAGTAAGCTAC40.1875293014533521No Hit
GTATGCCTGGACATTGATGCTAACATGACCATGCTTGGGATGTCTCTAGCTGGTCTGGGGATAGCTGGAGC40.1875293014533521No Hit
ATATCGTGAAAATGGCCATACTGCCCAATGTAATTTATAGATTCAACACTATTCCCATCAAACTACCATTG40.1875293014533521No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1875293014533521No Hit
CACCCTGTGTGTATCTTCATCTGGCTGTTTACTTATTTGTATCCTTTTCTAATAACGTTTGTAATAAACTG40.1875293014533521No Hit
GGGGATTCATTGGCTTCTTTGTGAAATTGATCCATATTCCTATTAATAACATCATTGTTGGTGGCTGAATA40.1875293014533521No Hit
CGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1875293014533521No Hit
GGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGTTTGCAGTGAGCCAAGATTGCGCCACTGCACTCCAGCC40.1875293014533521No Hit
ACACGACGCGGGACTGCTTTTTAAGAAATTTTTGTTTATGGATCTGATAAAATCTAGATCTCTAATATTTT40.1875293014533521No Hit
GCGTGGTGCCATCCCCAGGGGGCATGACCAGATGCGTCCCAAGATGTTGATTTTTACTGTGTTTTATAAAA40.1875293014533521No Hit
GTGCCAGTTCTTTCCATAATAAAAGGCTTTGAGTTAACTCACTGAGGGTATCTGACAATGCTGAGGTTATG40.1875293014533521No Hit
GGGTTTAGATTCTAGGCAGACCAAAAAATTTGAACTTCACTTTTGTTAGGACATGTGTCTCTCTCTTTGTA40.1875293014533521No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAGC40.1875293014533521No Hit
ATTTCCTAATTGATATTTTTGTGCCTAATAAAGGGGGTTTTCCTGTAAGTCGTATTAATAATTCTCTAAGG40.1875293014533521No Hit
CCAGTGGGGTGGTCAGTAGGAGAGCACGTTCAGAGGGAAGAGCCATCTCAACAGAATCGCACCAAACTATA40.1875293014533521No Hit
TCCCTACACGACGCGGGAGTCAGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1875293014533521No Hit
GGCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCATGTGTC40.1875293014533521No Hit
CCCCAGCACCAGAGCACAATGAGAAACGGCAGCGCCGACAGGAGCGGCGGCAGATGAAGCGGTTATAGCCA40.1875293014533521No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14064697609001406No Hit
CTCGTTGGGTCACGCCTTTCCCATTCCTGAAGAATAAGCGGAGTGCTTCCTGCAGCCGAAGACTCCATGCC30.14064697609001406No Hit
GTCCTACTTTTACAAATGTCCTGTGCGACTCCACCTCCAAACTGTGCCCTCAAAGGTTGTGTATAAGTACC30.14064697609001406No Hit
ATCCTATGTGGGTAATTGAGGGGTATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACCTCAACTG30.14064697609001406No Hit
GGTGGGAAGAGAGATTGTCCTGTGATTTCTACCCATTTCCTGAGGCCTGTGGAAATAAACCTTTATGTACT30.14064697609001406No Hit
GACTGTACCCATTATTTAGAAATCTAATGACAGATGTTATTAAGGGCTGCAAGGCACCCTATGAAAAGGCT30.14064697609001406No Hit
ATAAAGCACACTTATCACATAGCACAAAGGACGTGCCATGGTGCTTTCCCCAAAAGTTGTGTTGCTTTTAT30.14064697609001406No Hit
GCCTTATTGTATCGGTTTTATATTACCTGTTCTGCTTCACCAGGAGATCATGCTGCTGTGATACTGAGTTT30.14064697609001406No Hit
TGTAAAAATAAAATCTAACTCTCATTTGACAAGCAGAGAAAGAAAAGTTAAATACCAGATAAGCTTTTGAT30.14064697609001406No Hit
AGGTTGCAGTGAGCCGATATTGTGCCACTGCACTCCAGCCTGGGCGACAGAATGAGGCTACGTCTCAAAAA30.14064697609001406No Hit
CCATTGACTTAGAGGCAACAGGATTGATCATTCTTTTATAGAGCATATTTGCCAATAAAGCTTTTGGAAGC30.14064697609001406No Hit
GAAGCATGAAGGGAATCATAGGAAAAATGTACTGATCATATAATTAACATTATGTACTGTATATATCATTT30.14064697609001406No Hit
ACGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14064697609001406No Hit
CCCAGCTAATTTTTGTATTTTTAGTGGAGACGGGATTTCACCATGTTGGCCAGGATGGTCTCGATCTCTTG30.14064697609001406No Hit
GTTGCACTGTACGAAGTCTCTGAAATGTAATTAAAAGTTTTTATTGAGCCCCCGAAAAAAAAAAAAAAAAA30.14064697609001406No Hit
GCCTCGGTCTCCCAAAGTGCTGGGATTACAGGCGTGGGCCACGCGCCCAGCCTTAAATCTTAAACAGAGTA30.14064697609001406No Hit
ACTTAGTATTTTGAAATAGATTAATGTAGTCAGCAAACAATTCATGTATTACCTATAATAGATATCATACA30.14064697609001406No Hit
GAGTTGATTGTCTAGTGAGGAAGACAAGCATTTTTAAAAAATAAATTTAAACTTACAAAAAAAAAAAAAAA30.14064697609001406No Hit
GATATGATGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAATTTAAGTGGAAAAAAAA30.14064697609001406No Hit
GAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGG30.14064697609001406No Hit
CCCCCAGGGCTGCTTCCTGCCTGCACCTGCGGTGACTGTGAGCAGGATCCTGGGGCCGAGGCTGCAGCTCA30.14064697609001406No Hit
GACTCCACAGTGTATATATTTTGCCATTTATTTTCTAAAAATAAACAAAAATTGAAGGCAAAAAAAAAAAA30.14064697609001406No Hit
GGGCAGAACCTGAGCCCTCCCAGTGCTTGCCTTCAGCCACCTGGCTCCCCTCCTGGGAGGCCTCTGCTGGA30.14064697609001406No Hit
CTTGCTGTTGTACACAGGGTATTTCTAGAAGCAGAAATAGACTGGGAAGATGCACAACCAAGGGGTTACAG30.14064697609001406No Hit
CTTCCGATCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14064697609001406No Hit
GGAGGCCGCCTCCTCGGGGCTCCAGGCTGGCTTGCCCGCGCTCTTTCTTCCCTCGTGACAGTGGTGTGTGG30.14064697609001406No Hit
AGAGGAGAGTGAAGAGGAAGAGGTCGATGAAACAGGTGTAGAAGTTAAGGACATAGAATTGGTCATGTCAC30.14064697609001406No Hit
TATCTTCATTAAGAGTTTTTGGAAAACTCATCAAATTCGATGAATACATTTTCTTCATAACCCATTTGGAA30.14064697609001406No Hit
GGTTACATCTTTGACCTTCTTAAGAATGAGTGAAGACATTTGTTTCATTTAGCATTGGTTGAGCCTCAGAT30.14064697609001406No Hit
CACGACGCGGGATTATAATCATTATAAAACAAAATTAAGTAAAAGGTTATGACCCTTGAAAATTTTGATAT30.14064697609001406No Hit
CAGGCGATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCAGCCAACA30.14064697609001406No Hit
CACGACGCGGGGCTGTTTTCAAGAACTTTAAAATAAAATATGAATATTCAAGCTGTGCTTCCTGTTGTGTA30.14064697609001406No Hit
GTCACAATATTTTCTCTAGCAACTAATGAGATGTGGCGATCAGGGGATAGTGAAGTTTACCAACTGGGTGA30.14064697609001406No Hit
GCCCTTGGAATTGCTTTTGTCGAGGTTTTGGGAATTGTCTTTGCCTGCTGCCTTGTGAAGAGTATCAGAAG30.14064697609001406No Hit
GGGCAACTGAGAAAGAACAGAGAAGTGACAGCTAGGGGTTGAGAGGGGCCAGAAAAACATGAATGCAGGCA30.14064697609001406No Hit
CGACGCGGGGGGAACTGAACAACCTGCTCCTCAGTGACTACTGGGTACATAACGAAATGAAGGCAGAAATA30.14064697609001406No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC30.14064697609001406No Hit
GGCTGCTCACCATACGGCTCTAACAGATTAGGGGCTAAAACGATTACTGACTTTCCTTGAGTAGTTTTTAT30.14064697609001406No Hit
AGGTTTACCAGTAGAATCCTTAGGTTGATGTGGGCCATACATTCCTTTAATAAACCATTGTGTACAAAAAA30.14064697609001406No Hit
GTTGAGTCTCTGAACATCCTAAATATTGGTTTGTTATTCTTTTCCAGAAAGAAAATGAATTTGACTGGTTC30.14064697609001406No Hit

[OK]Adapter Content

Adapter graph