FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25118.73134328358209No Hit
GCTGATAAGATTCTCTGTAGAGAAAATACTTTTAAAAAATGCAGGTTGTAGCTTTTTGATGGGCTACTCAT352.6119402985074625No Hit
TGATGATGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCTTCCAAGAATGAGGCAAACTGAATTG332.4626865671641793No Hit
CTGTGAGTCCAGCGGCGCAGGGTGGTACCAGGAAAGAGGATATTCTAGGCTTTGCGTGCTGCTAGCTGGGT302.2388059701492535No Hit
CTTTAATATAATGTTAGAGATGTCTTCATAGGCAGTATGGCTATCTTTGCCACAGAAACATAAGTAAAATT251.8656716417910446No Hit
GTTCGAAACCAGCCTGGGCAACATAGTGAGACCTCCTCTCCGCAAATAAAAAAAAAATTACCTAGGCATGG221.6417910447761193No Hit
ATGTATCAGTAACTCACCTTATTGCAGGAGAAGTTGGTAGCAAAAAATATTTAGTTGCTGCAAACCTGAAG221.6417910447761193No Hit
CAAGTGAAAAAACAAAATTGCCTGAATTGTACTGTATGTAGCTGCACTACAACAGATTCTTACCGTCTCCA211.5671641791044775No Hit
CCAGTGTACTGCTTTCAACTTAAATCACTGAGGCATTTTTACTACTATTCTGTTAAAATCAGGATTTTAGT191.4179104477611941No Hit
CTCTGGCGGTGGCGGCCTGGGGCGGCGCGACGGCTGGTGCGCAGGTACACTGATGCTGAAGTACTATGAGC191.4179104477611941No Hit
GCACGTTTCCCCCAAACCCTGGACTGACTGCTTTAAGGTCCGCAAGGCGGGCCAGGGCCGAGACGCGAGTC171.2686567164179103No Hit
CTCTAATATTTTTAAGCCCAAGCCCCTTGGACACTGCAGCTCTTTTCAGTTTTTGCTTATACACAATTCAT171.2686567164179103No Hit
AATTAAGCCGAAGAAGCCTGGGAATCAAGTTTGAAACAAAGATTAATAAAGTTCTTTGCCTAGTAAAAAAA171.2686567164179103No Hit
AATTAGAAGAAGAAAGCGTTGCAGAAGATGATGCAGAATTAACATTAAATAAAGTGGATGAAGAATTTGTG161.1940298507462688No Hit
GTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCA151.1194029850746268No Hit
GGCTGATCAAAGAAAGAAGGCATACGCAGATTTCTACAGAAACTACGATGTCATGAAAGATTTTGAGGAGA141.0447761194029852No Hit
GCCATATACCTGGAGGGAATGTGCTTTGTCACACCAAAGAGGATTTTTTTTTCTTCAAACTTGTATGTTGC141.0447761194029852No Hit
GTTTTAGACTCTGAAGGATAATAAATGGATGATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141.0447761194029852No Hit
TTATAAAATAATGTGTGTAATCCTTGTTGCTTTCCTGATACCAGACTGTTTCCCGTGGTTGGTTAGAATAT141.0447761194029852No Hit
GATTATGCTTGTGTGAGGCAATCATGGTGGCATCACCCATAAAGGGAACACATTTGACTTTTTTTTCTCAT130.9701492537313432No Hit
TTGTACAAGTCTGGAAAAAGAAAAAAGGGAAGAAGTTCATAAATATGTACAAATGATGGGCGGACGAGTAT120.8955223880597015No Hit
GTACCTGGACCTAGCACCCAGCACCTTTCTCTGAGCTGAGTGGCTGGAGTGGTCAATAAAGCCACATGTGC120.8955223880597015No Hit
CAATAAACAAGGTTTCCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.8208955223880596No Hit
GGATACACCAACATCAGCTGGACCAAACTCCTTCAATAAAGGAAAGCATGGGTTTTCTGATAACCAGAAGC110.8208955223880596No Hit
CGACGCGGGTGGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.8208955223880596No Hit
CTTGTTACATGTATTACTTGGTGTATCGATAATCATTTAAAAGTAAAGACTCTGTCATGCATTTTTAAAAA110.8208955223880596No Hit
GGGCTGTGCTGCTTTTGAGCCTCAGACCCCAGGGCAGCATCTCGGTTCTGCGCCACTTCCTTTGTGTTTAT110.8208955223880596No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAA100.7462686567164178No Hit
GGATGAAGAGGGGCCCTTGTGTTGCCCCCACCCTGGCTGCCTGCTAAGGGCCCATGTGATCTGCCTGGCAG100.7462686567164178No Hit
CTCTTTCCCTACACGACGCGGGCAGCTGATTCCAGGCGAATTCTTCTCTGATGGGCGGGCGAGGGTGTGTT100.7462686567164178No Hit
CCATAGTACTGTGGCTTCAGATTTAGTACCTATGAACAGATGTACAAGACATTTATTACACTTTTTACCAA90.6716417910447761No Hit
GTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCAT90.6716417910447761No Hit
GTTATATGTAAGATCTGATTTGCTAGTTCTTCCTTGTAGAGTTATAAATGGAAAGATTACACTAAAGAAAA90.6716417910447761No Hit
GTAAAATCCAGAACTTGGACTCCATCGTTAAAATTATTTATGTGTAACATTCAAATGTGTGCATTAAATAT90.6716417910447761No Hit
CTCCGGAGGCGGCCTGGGTGGGTGAGCCATGGGGCAGTTGACCTGGGTCTTGAAAGAGTCGGGAGTGACAA80.5970149253731344No Hit
CTGGGAGGAGATAAGAAGAGAAAGGGCCAAGTGATCCAGTTCTAAGTGTCATCTTTTATTATGAAGACAAT80.5970149253731344No Hit
CCTCTGTTGTTCCTAATGCTCAAAATCAAGGGGCCTCTGAAGGTGTACTTGGCTAAATGTAAGACATCTGG70.5223880597014926No Hit
GAAATAATTGTTGTTCTGTTAACTGAATACCACTCTGTAATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA70.5223880597014926No Hit
GAAATGAGGGACTGGGATCTGCACAGCCATTAAATTATAAATCTGGAAAAAAAAAAAAAAAAAAAAAAAAA60.44776119402985076No Hit
TAAAGGAACTGGACAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.44776119402985076No Hit
ATATGGTACAGCTCTTCATTTACTTAGGCCTTTGAAATATCTTTCAATAAAGTTTATAATTTTCTCCAAAA60.44776119402985076No Hit
GGCTTAAATAAATAACTTAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.44776119402985076No Hit
AGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAAAAAAAAAAAAAAA60.44776119402985076No Hit
AATAAAGACTGTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3731343283582089No Hit
CAAGTGAAAAAACAAAATTGCCTGAATTGTACTGTATGTAGCTGCACTACAACAGATTCTTACCGTCTCCC50.3731343283582089No Hit
TTATTGACCCTATCTTCTCATTGTTTGTTTGTAAGTTTGAATTTGTATTAAAAAGCCTGCATTCTGAGCTG50.3731343283582089No Hit
ACTCTGGATGCTGTAAGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3731343283582089No Hit
GAAATAAAGGCTTACTTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
AGATAGCGCCACTGTACTCCAGCCTGGAGGACAAGAGCGAAACTCCCTCTCAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2985074626865672No Hit
ATCCAACCCCACGCTTGCAGTGACTCAGAATGATAATTATTATGACTGTTTATCGATGCTTCCCACAGTGT40.2985074626865672No Hit
GGTAAGGTTTCTAGGAGGTCTGTTAGGTGTACATCCTGCAGCTTATTGGCTTAAAATGTACTCTCCTTTTA40.2985074626865672No Hit
CGGTAATATGTATAAACTTACATCACGACTTTCTCTTAACAGCTGTTGTAAAGTTTATTACTTTATGTACA40.2985074626865672No Hit
TGTCTGTACTGATCCTCAACCAATAAAATCTCAGTTATGGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
CACCACAGAAAAAAAAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
GGAGTTTGAGACTAGCCTGGCTAACATGGTGAAACCCTGTCTCTACTAAAAAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
TTCCCTACACGACGCGGGCACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAA40.2985074626865672No Hit
CCATTTTTGTTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2985074626865672No Hit
AAGAGGATGAGAAGGCAAAGCCCCCAAGAGAGAAGGCCACTGATGAGGGCAGGAGAGAGACGACCGTGTAC30.22388059701492538No Hit
TTATTACAGAGAGAGAAGCAGAGAACGAGAGAGGCACCGGGATCGTGACCGAGACCGTGACCGAGAGCGTG30.22388059701492538No Hit
TCCCTACACGACGCGGGATGGTTTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTAAAAAAAAA30.22388059701492538No Hit
TCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGGAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTT30.22388059701492538No Hit
ATCTGCTTGTTGATCAGTGTTGAAGAAAGTGCAAAAGGAACTTTTATATATTTGACAGTGTAGGAAATTGT30.22388059701492538No Hit
GTCTGTCAAGACCAAGATTCAGAAGCTGGGCAGGGCTATGCGGGAGATTGTCTAAAAGTGTTGGTTTTCTG30.22388059701492538No Hit
CTCTTATATTATGTTTTGTGGAAATCAGCACACAACCACAATGACATTTAAGCACAGGATCATTATTAGTC30.22388059701492538No Hit
ATCCATGGTTAATAAATGTGGTTTCTACATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22388059701492538No Hit
GGTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22388059701492538No Hit
CTCTGGCGGTGGCGGCCTGGGGCGGCGCGACGGCTGGTGCGCAGGTACACTGATGCTGAAGTACTATGCGC30.22388059701492538No Hit
ATTGCAAACCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22388059701492538No Hit
GGGGTGACCAGTCCCGGAAGAGAGCTGGGGATGAGTTGGCTTATAATAGCTCGTCAGCATGTGCAAGTTCC20.1492537313432836No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG20.1492537313432836No Hit
CTCTTCTTACTGGAGGCTATTTAAATAAAATGTAAGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
CTCTATGCCTGCACTGGGAAGGAAACAAATGTGTATAAACTGCTGTCAATAAATGACACCCAGACCTTCCG20.1492537313432836No Hit
GTATTATTTATTGAACTTTTTGTTTGAATACATGTGCCTATTTTTGCATAAATGTAAAAATGTAATTTTAA20.1492537313432836No Hit
GACTAAACCCTCCACTCCTGATTCTCAAGAGTGTATCACCTGTCAGCAAAATGAATAGTGGGATATTTTGG20.1492537313432836No Hit
CAACAAAATTCTTTGGAACAACTGGAACCTGACTATTTTAAGGACATGACACCAACTATTAGGAAAACTCA20.1492537313432836No Hit
CTCTGGCGGTGGCGGCCTGGGGCGGCGCGCCGGCTGGTGCGCAGGTACACTGATGCTGAAGTACTATGAGC20.1492537313432836No Hit
TTCCCTACACGACGCGGGGGCACTTAAGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
AGTTTCATTAGTGTTGATTCTCAATGTTGTCTGCTGATGTTGCTAAAGCAGCCACACAGTAGCATTACAAA20.1492537313432836No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAA20.1492537313432836No Hit
ACAGAGCAAGACTGTCTCAAAAATAAATAAATAAATAAAATTGTTTAAATGAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
CCTCTACTCTTTGAGACATCACTGGCCTATAATAAATGGGTTAATTTATGTAACAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
ATTCTGAGCCATGCCATTTTTACCTTATGTCTGCTAGAAAGTGTTGTAGTTGATTGACCAAACCAGTTCAT20.1492537313432836No Hit
GTCATCCTCCGTCTACCAGAGCGTGCACTTGTGATCCTAAAATAAGCTTCATCTCCGGGCTGTGCCCCTTG20.1492537313432836No Hit
TCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCACAGCTGCCCCC20.1492537313432836No Hit
TTCACAAACTGAAAGAAAAAGCGAAGAAACGGAAGGGTCGCGGCTTTGGCTCCGAAGAGGGGTCCCGAGCG20.1492537313432836No Hit
CCATAGTACTGTGGCTTCAGATTTAGTACCTATGAACAGATGTACAAGACATTTATTACACTTTTTAACAA20.1492537313432836No Hit
GTATAAGGCACCCCAGTCCAGAGATAGAGATACTATTGGCGATGGGAAAACATCAGGAGCACACCAACTGG20.1492537313432836No Hit
GTGTAAAAGAGAATACCAATTTGGCAGGAAGAGAACATATTCTGCATCCCTCACTGAATTCAGTAATAAAT20.1492537313432836No Hit
TCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCGCGGCGGCCCCC20.1492537313432836No Hit
TCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCTCTGCTGCCCCC20.1492537313432836No Hit
GGTTGTGACAGACTTATAAAATCTTTTTAAAAAATAAAGCTATAATTTATATTAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
ATCTAGCACCATGACGGAACTAGAGACAGCCATGGGCATGATCATAGACGTCTTTTCCCGATATTCGGGCA20.1492537313432836No Hit
CTGTGAGTCCAGCGGCGCAGGGTGGTACCAGGAAAGAGGATATTCTAGGCTTTTCGTGCTGCTAGCTGGGT20.1492537313432836No Hit
CTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
GTGTCTGGAAATTTTTTTTTCTAAGAAAAACTATTAAAGTACTTCCTAGTAGGAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
GCATAAGTGGCAATCATAAAAAGTAATAAAGGTTCTTTTTGACCTGTTAAAAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)