FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33227.347611202635914No Hit
GGCATCTACGGCTCAACATTTTTTGTAACCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTT514.200988467874795No Hit
GACTTTACAGTGTGTTACATATGATTGTATTTACTTTAGAATTTTTAGAAAGCATACCATTTTCAGGTTTA362.9654036243822075No Hit
GCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCTGGCGTGGTGGTGCACACC282.3064250411861615No Hit
GTAACTCTACTGCCCACCAAAATTAGTTTTGTGTTCTATGCTCAAATACTTATATTAAAACGCTGGTAAAT272.224052718286656No Hit
ATTACACTGTACTACCCAAGAAGACTGTTTATTGTGAAGCATTTACCTTTCAAAAAATCATTACATTTCTA241.9769357495881383No Hit
GTGCTGTTGGTGCAACAGATGATTCAATAAATGTCTACAGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA201.6474464579901154No Hit
CTTCTAGAATGATACCCAAACTCCTGGAGTGGGAGTGGGGAATGCCTTCTACGTACACACTGTTCTACTGT191.5650741350906094No Hit
AACTCAGGAACGCCCCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171.400329489291598No Hit
CCTGTTTTCTGACTAGTTTAAAGATGGATTTGAAAATGGTTTTGAATGCAATTAGGTTATGCTATTTGGAC151.2355848434925865No Hit
GGCTGGAGTGCAGTGGTGCAATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGTGATTCTGCTGCC151.2355848434925865No Hit
CTTCAAGTCTCTGCAGCCTGCTCCACATTTGGGCACAGGAGGACTCAGACAGGCACTGTGTGGGCACGAGG141.1532125205930808No Hit
GACTTTGGCCTCGACCTGCAGCCCTGCATCGACCTCATTTGCCATCGAGGCCTTGGCCAAGGCGACCTATG141.1532125205930808No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT141.1532125205930808No Hit
AGTCCTCAAATCAGGAAGAAACATCAAAGACTATGTCCTAGTGGAGGGAGTAATCCTAACACCCAGGCTGG131.070840197693575No Hit
GTCCTAAATATTAATATCCTGTAATAAAGCTCTTTAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.8237232289950577No Hit
GATTAAATCAATAAATAACTGAAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.7413509060955519No Hit
GTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.7413509060955519No Hit
GCTCACCCACCAGCTGCTGGAGCTGAGGCCCTGGTGGGGGAAAAGCAGCCACCACATGATGCGGGAGAACC90.7413509060955519No Hit
GTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGACAGTTGAAAATCCTAAGTTCAG80.6589785831960462No Hit
CATGTAACCCAGCAGCAGTTTGCTTCTGTTGTCCACTGATTAATCAGCCTATGTGCCTGACACTGGTCTTG80.6589785831960462No Hit
GGTAAAGAGTTCTATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.6589785831960462No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.5766062602965404No Hit
GTATTACATCTTGACTTGTAATCAATTATGAATATTTCTTGATATTTAATGTATAGGACATTTATTTATAC70.5766062602965404No Hit
GGAGGACTCAGACAGGCACTGTGTGGGCACGAGGTTTTACAGGGGTGGTCAGACCTCAGGCTTTAATGAAT70.5766062602965404No Hit
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTATAAAGAATAA60.4942339373970346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.4942339373970346No Hit
GTACTGTAATATAATGAGGCCATCTAGATGTTCCAGAATATGTTTACATGGTGTTATTTACATTATTAAAA60.4942339373970346No Hit
GATTTCTTCCAAAGTCAGAGAAAACTACTTCAAAGAAGCCCTTCTGAATTCTGTAATATGGTTGAAAGTGT50.41186161449752884No Hit
TTATTAAACTACAGGCTGTAGATTTCTTAGTTCTCACAGAACTTCTATCATTTTAAACTGACTTGTATATT50.41186161449752884No Hit
CATTTGTACTTAAGTCATTCCATCTTTCACTCAGGATGAATGCGAAAAGTGACTGTTCACAGACCTCAGTG50.41186161449752884No Hit
GTGCCGAACGCTGGCCACTGGATCCACGCTGACCGCCCACAGGACTTCATAGCTGCCATCCGAGGCTTCCT50.41186161449752884No Hit
GTTATGAGGGAGGAAGTCAACTGCTGTTCAGCCTCAGAATAAAGGTGCCGTTCACTGGCTCAAAAAAAAAA50.41186161449752884No Hit
GTCAAACAAACAAAACCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3294892915980231No Hit
ATTCTGCAGTTGTTACTTAGGAAGCCTGGGGAGGGCAGGGGTGCCCCATGGCTCCCAGACTCTGTCTGTGC40.3294892915980231No Hit
CTATAAAGTGTTTTTAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3294892915980231No Hit
CCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCGAGTATCTCTTCGAAAGGCTCACTC40.3294892915980231No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG30.2471169686985173No Hit
TACTAGATATGTTTGTCAATAAACTTATGACGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2471169686985173No Hit
GTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGACAGTTGAAAATCCTAAGTTAAG30.2471169686985173No Hit
AAACTTTCTTTGTACTGTGATTTCCTTTTGGGTGTATTTTGCTAAGTGAAACTTGTTAAATTTTTTGTTAA30.2471169686985173No Hit
ACCTTGGCCCGAGCCCCTCCGTGAGGAACACAATCTCAATCGTTGCTGAATCCTTTCATATCCTAATAGGA30.2471169686985173No Hit
TCTTAATACTCACCAATAAATTCTACTTCCTGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2471169686985173No Hit
GAGGTGAAGTTGGAATGGACTTTTTCTCTTTTTAGTGAATCAACAGTTCATGCCTCAATTTTGGGTAACCC30.2471169686985173No Hit
CAGTAATGCAGGGGCTGTTGGGTGCTTCCTGCAAATCCCTGAGAGGGCAGAAGATAGCTTCTGTTAATTCA30.2471169686985173No Hit
AATTACAAGTTTCAGTAATAGCTGAACCTGTTCAAAATGTTAATAAAGGTTTCGTTGCATGGTAAAAAAAA30.2471169686985173No Hit
GTATATGTGTATCAAAATATCATATGGCCTCAGAATATGTACAGCTATGATATATCAATTAGAAATACAAA30.2471169686985173No Hit
GAACAGATTTTGCAGTTGGTGGGGTCTCAATAAAAGTTATTTTCCACTGAAAAAAAAAAAAAAAAAAAAAA30.2471169686985173No Hit
ACTTCATAGCTGCCATCCGAGGCTTCCTGGTCTAAGAGTTGCTGGCAAGAAGATGGCCGGGCGTGGTGGCG30.2471169686985173No Hit
GAATTAAGCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2471169686985173No Hit
ATTTTACATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2471169686985173No Hit
AAGTTATCATGTCCATCCGCACCAAGCTGCAGAACAAGGAGCATGTGATTGAGGCCCTGCGCAGGGCCAAG20.16474464579901155No Hit
GGCTGGAGTGCAGTGGTGCAATCTCGGCTCACTGCAACCTCTGCCGCCCAGGTTCAAGTGATTCTGCTGCC20.16474464579901155No Hit
GTACTTTTCCCATTTGTAATTTGTAAAATGTTCTCTTATGATCACCATGTATTTTGTAAATAATAAATAGT20.16474464579901155No Hit
GTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGACAGTTGAAAATACTAAGTTCAG20.16474464579901155No Hit
CGCCTGGAAACGTGCCAGGACACTGTCCTGGTGCCCAGCCCAACGTGGTCCAACGTTTTTTTATAATAAAA20.16474464579901155No Hit
CTCTAACAGCCCTTTCATGAAGGTAATGCTAGTCTTCTGTCCATCAGTGCCATTTCCTGTAGAACTAAAGG20.16474464579901155No Hit
CGCCTGGAAACGTGCCAGGACACTGTCCTGGTGCCCAGCCCAACGTGGTCCAACGTTTTTTTATAATAAAG20.16474464579901155No Hit
TTCCCTACACGACGCGGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16474464579901155No Hit
CTGTTCGTCTGCTGTCTGTGTCTTCTATTTGGCAAACAGCAATGATCTTCCAATAAAAGATTTCAGATGCG20.16474464579901155No Hit
ATATTGCTTTGTAATGGTTTGTGATACTTGAAACATCACGGTGCATAATAAAGTAGGTCTGCAGTAAAAAA20.16474464579901155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAA20.16474464579901155No Hit
CTGGTTTTCATGCGCTGTTTTTGTTTTAAAGTTATGAAGAAAAAAGTCAATAAAATTCTAAAAAAAAAAAA20.16474464579901155No Hit
CTCCATGAATATGTATAATTGTTATTAGTCAGTTAAAACAAATAAAACTAAAAATGTTTAACGATAAAAAA20.16474464579901155No Hit
TTCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16474464579901155No Hit
GTAAAACGTAAAGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16474464579901155No Hit
TCACCTACCTGCCAGCGGGGCAGTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCACCCTGCCTGCACCA20.16474464579901155No Hit
TTATTTTAGTGTGTAAAATTAATGTAATAAAGTGAACTTTGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAA20.16474464579901155No Hit
AACTACAGCATCATTGCATAATTCTTGATGGTAATAAATTTGAATAATCAGATTTCTTACAAAAAAAAAAA20.16474464579901155No Hit
CCGGTGAATGTGCCTCTGGTGGCTGCTGAGAAAAATACACTGTGCAGCTCAAAAAAAAAAAAAAAAAAAAA20.16474464579901155No Hit
GTGCCGAACGCTGGCCACTGGATCCACGCTGACCGCCCACAGGACTTCATAGCTGCCATCCGAGGCTTCCG20.16474464579901155No Hit
GATCAAGAAGCCGGACTACAAGGTCGCCTACGTGCAGCTGGCCCATGGACAGACCTTCACGTTCCCAGATC20.16474464579901155No Hit
TGTCCAGTGTCTGCTTGCCATCCTGCCAGAACAGGCCCTCAAGCCCAAGAGCCATCCCAGGCCTGTTCAGG20.16474464579901155No Hit
GGGTTCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGTAGACTTATTGCGGCCTGGTTT20.16474464579901155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)