FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67722.182175622542594No Hit
GCTGCTCAGGAGGCCGAGGCAGGAGAATCACTTGAACCCGGGAGGCGGAGGCTGTAGTGAGCCGAGGTTGT411.343381389252949No Hit
AATTACTTGTGAAAAACAACACATTTTAAAGATTACGTGCTTCTTGGTACAGGTTTGTGAATGACAGTTTA351.146788990825688No Hit
ACAAGCGAGTTTTTAAACAAAATGACTGAAGCACAGGAAGATGGCCAGTCAACTTCTGAATTGATTGGCCA311.0157273918741807No Hit
GCCTTCCACCGGCACTCATGACGGCCTGCCTGCAAACCTGCTGGTGGGGCAGACCCGAAAATCCAGCGTGC311.0157273918741807No Hit
GTATTTTAAAAGCTCAAAGTAGAAGAGGAGTCACTAAGCCCTAACAGCTTGTCCAAAGATTTAAATTCTTA300.9829619921363041No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG290.9501965923984272No Hit
GTCCTAAGTCTGTGGCTCGTATCGCCAAGCTCGAAAAGGCAAAGGCTAAAGAACTTGCCACTAAACTGGGT290.9501965923984272No Hit
ATGCAGAGATTACAGGCATGAGCCACCGCACCCGGCCAGCCCCAACACTATTACTGAAGGTAAATAAAAAT260.8519003931847969No Hit
GAATCAGAAATGAAGATGAGACTACCAAGACGAGCCAAAACCGCAGCACTAGAAAAAAGTAAACTTAACCT260.8519003931847969No Hit
AGTGTATCTTTCACTGATTTCTGAATCATGTTCTAGTTGCTTGACCCTGCCACATGGGTCCAGTGTTCATC240.7863695937090431No Hit
GAAAATGGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGACTGCACCACTGCACTCCAGCCTGGGT240.7863695937090431No Hit
GGTGCTCACATGCACCTCACTAGGGTTTGTGACCAGGGTCTGGATGAGCTTGAATTTGAATGAATTGAGTT230.7536041939711665No Hit
GTGCTTATTGACTACAAGAGGAAATTCTACAGGATTAGGATTTCTAAGACTACTATAGGAATTGGTTGGCA220.7208387942332897No Hit
GTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCT210.6880733944954129No Hit
GTATGACTGATGATCCTCCAACAACAAAACCACTTACTGCTCGTAAATTCATTTGGACGAACCATAAATTC210.6880733944954129No Hit
CCCCTTCTCTACTAAAAAAATACAAAATTAGCCAGGCATGGTGGTGCATGCCTGTAACTCCAGATACTTGG200.655307994757536No Hit
GGTTGACAAGGTCACAGGCAGGTTTAATGGCCAGTTTAAAACTTATGCTATCTGCGGGGCCATTCGTAGGA200.655307994757536No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCC190.6225425950196593No Hit
GGTGTATTAGTCTGTTCTCACACTGCTATGAAGAGATGTCTGAGACTGGGTCATTTATAAAGAAAAGAGGA190.6225425950196593No Hit
GATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGACGATGTCGATACCAA180.5897771952817824No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT170.5570117955439057No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT170.5570117955439057No Hit
GGTAAACGCAGTGTGGGAAAGGAATGTGGAATGAGAACTTGGTGGTTCACCGCTGTACTATTTGTGTAAAT170.5570117955439057No Hit
GGCTTACACCCTTCGTGGACAGGCGAGGCTCTGGTGCTTACTGCACAGCCTGAACAGACAGTTCTGGGGCC160.5242463958060288No Hit
AGAAAAGGCTTGCCAATCTATTTATCCTCTCCATGATGTCTTCGTTAGAAAAGTAAAAATGCTGAAGAAGC160.5242463958060288No Hit
GGAAACCCAGAAACATTTTACGTTGCTTTTACTTCTGTAGTGTAGATTGCCCCGGCCCCTCTCTGAGCCCT160.5242463958060288No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC150.49148099606815204No Hit
CCTTTGTGATTCTCCTCATCAGAATCTCTCAAGACCTCTTCTGGAAAACAAACTTAAAGCATTCAGTATTG150.49148099606815204No Hit
GGTAATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCTGGTTAAAAGATGCACTA140.45871559633027525No Hit
ATTACAAATTGGATGGAACTGTGTTTTTTTCTGCTTTGTTTTTTCAGTTTGCTGTTTCTGTAGCCATATTT140.45871559633027525No Hit
CTCCAGGCCCTGCCCCCTCCCACTCTTGAAGAGGAGGCCGCCTCCTCGGGGCTCCAGGCTGGCTTGCCCGC140.45871559633027525No Hit
ATACTGAAATGTGAGCCACTTAATAAAGGTTCATATGTTCATATTAAAAAAAAAAAAAAAAAAAAAAAAAA140.45871559633027525No Hit
GGCATCTGGGAGGGCCCTGCCCCCATGGGCTTTACCCTTCCCTGCGGGCTCTCTCCCCGACACATTTGTTA130.42595019659239847No Hit
GAAACCCGGTAGTCTAGAACTAATCAGATTACTGATTTTAGGACACAGCACCAGATGAATTGTTGTATATG130.42595019659239847No Hit
GTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCC120.39318479685452157No Hit
GTGCTTGTCTGTTTAGTTCTGTGTATAAATGGAATGTTGTGGAGATGAAAAAAAAAAAAAAAAAAAAAAAA120.39318479685452157No Hit
GTACATTCCTTTTTCCTTTTTCTGCCTCTTCCCTCAATAGTTGCCTTTTAGTGCTGTAATAGTTAAATCCT110.36041939711664484No Hit
AAGCTGCAGAACCAACGAGGTGGCCGAATCTTCCTTCAGGATATCAAGAAACCAGACTGTGATGACTGGGA110.36041939711664484No Hit
GATACAAGTCTTAACCACAGTGTAACTTACAGTTTCCTTTAAAAAAAAAAAGTAAACCTGGCAGCTATAGA100.327653997378768No Hit
TGTCCTCCCCCTGCCACATCAGTGACTGCTTTATTCTTTTCCAATAAAGAAGTGCACGTGTCAGAGCTGGA100.327653997378768No Hit
GCATATTGTAGATGCACTTTGCAGCAGTTGGAAAAGAAAGTGTTGTGTGATTTGATTGAAATAAAACTAAA100.327653997378768No Hit
GGATAAATGCTTGAGGTGATGGATAGCTCATGTATCCTGATGTGATTATTCCACATTGCATACCTGTATCA100.327653997378768No Hit
GAGAACTACAAGAAATTCTATGAGCAGTTCTCTAAAAACATAAAGCTTGGAATACACGAAGACTCTCAAAA100.327653997378768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.327653997378768No Hit
TTTGTGGACATTGGGGTCACAACTTTTGGAGAGACAAGTTTAAGAAGTTTGATGATAAATATCTGCGGAAG100.327653997378768No Hit
TATATGAGCAGGCTGTGGCTTCATTTCCCAAGAAGCAGGAGTGACTTCAGTCATCCCAGCAATCGCTTGGT100.327653997378768No Hit
CACTGGTGGTCCATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTACAAAGACAGGAAAC100.327653997378768No Hit
AACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAGGGGGCCTTCCAG90.2948885976408912No Hit
GGGCAGAGACTTGTCACATCTTTGTCCCCAGCAAAGGCTACATGTTACCTCCTTCAGTTGATAATAAACCT90.2948885976408912No Hit
ACTCAGACCTGTGTGTGTTTGTTTTGTTTTTGTTTCATCTTTAGGAAAAAAGGGGTGCTGGAAACATTCTC90.2948885976408912No Hit
GGGTTTGAAGGGTGTTAGCATCCATTTCAGGGGAGTGTGGATTGGCTGGCTCTCTGGTAGCATTTTGTCCT90.2948885976408912No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.2621231979030144No Hit
GTTTTGTTGACATTCTGAATGCTTCTAAGTAAATACAATTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAA80.2621231979030144No Hit
CTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAAC80.2621231979030144No Hit
GGCTGTTGAGAATTCTTTGCCACTTTTCCCACCCCAAAACAGCATGGGGCCTGACATCTTCTGCCCTGGTC80.2621231979030144No Hit
TGCGAGACCCTGACGACCAGTACAAGCTCACGGAGGACACCCGGCAGCTGGGCCTCGTGGTGTGCCGGGGC70.22935779816513763No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT70.22935779816513763No Hit
AGATTGGGTACTTAGTTTCCTGTTACTGAGTTAGCTCTACTCTTTTGGACCAAAGCAACATGAGAGCAAGT70.22935779816513763No Hit
AAATAAGGATCACACTGTATTAGGGGTTATGGCAACATTATTGAATTTTTTATGTACATAAAGCCATATGT70.22935779816513763No Hit
GTATTGCCTCTATTCTGTAAAGTTCTGTACATAGTTCCCAAGCTTCTGTAGGGGGTGATTTTTGCTCTTGT70.22935779816513763No Hit
CATGTAAGTGGAATAAAAGTTTTATGAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.22935779816513763No Hit
GCTCTGTTTTCACTGTTCGTCTGCTGTCTGTGTCTTCTATTTGGCAAACAGCAATGATCTTCCAATAAAAA70.22935779816513763No Hit
TTCTAGATTGTGGATCTTCAGATAAATTCTGCCATTTTCATTTCACTTCCTGAAAGTCAGGGTCGGCTTGT70.22935779816513763No Hit
CATGCAGTTCATTATTTTGTGGTTCTATTTTACTTTGTACTTGTGTTTGCTTAAACAAAGTGACTGTTTGG60.19659239842726078No Hit
GAGGCAGGAGAATCATTTGAACCTGGGAGGCGGAGGTTACGGTGAGCTGAGATCACGCCATTGCACTCCAG60.19659239842726078No Hit
GTCTTGTACATACATACTTTTTTATGAGCTATGAAATAAAACATTTTAAACAAAAAAAAAAAAAAAAAAAA60.19659239842726078No Hit
GAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAAAAAAATGAAACTTTTT60.19659239842726078No Hit
AATTTATCTAGTGTTTATAAAATGTGATTTGTAATAAATGTTGTTAAAATGAAAAAAAAAAAAAAAAAAAA60.19659239842726078No Hit
GGCAGATGCGGGTGTGGAGGTGTGGGCTGAAGGTAGTGACTGTTTGATTTTAAAAAGTGTGACTGTCAGTT60.19659239842726078No Hit
GCTAAGATGCCATGTGCAGGTGGATTCCATGCCGCAGACAAGAAATAAAAGCTTTGCAAAGACAAAAAAAA60.19659239842726078No Hit
TTCCCTACACGACGCGGGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19659239842726078No Hit
TGTGAAAACCAACCCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19659239842726078No Hit
ATTATACTTTGTTAAAAAGGACAAATTGTACATTTTTAGAATGTTTTTATGAGTAAATTTAATGTACTGAA60.19659239842726078No Hit
GTAGAAAGGTTTATATTTCATCTTAATTCTTTTGATGTTGTAAACGTACTTTTTAAAAGATGGATTATTTT60.19659239842726078No Hit
CTTAAGTGCCTGTTTGAGTTTACTGAAACAGTTTACTTTTGTTCAATAAAGTTTGTATGTTGCATTTAAAA60.19659239842726078No Hit
GAGCGTGTGGCTGTGCCCCTTCTCTTTAATCTCATTTAATTTTTATTAAACATGCTCAGTACCAAAAAAAA60.19659239842726078No Hit
GAATCGAAATCAACTTTCCAGTAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTAT60.19659239842726078No Hit
CTATGAGGAGGGCCCTGGGAAGAATTTGCCATTTTCAGTGGAAAACAAGTGGTCGTTACTAGCTAAGATGT60.19659239842726078No Hit
TGGACCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.163826998689384No Hit
GACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAA50.163826998689384No Hit
TCCTTAGCCTGGATTTCCTTCTAACATGTTATCAAATCTGGGTATCTTTCCAGGCTTCCCTGACTTGCTTT50.163826998689384No Hit
GTGGTGCCCATGAGCCCCTGCTCCTCAGCCAGGCCCGTGGCGCCGGCTCATGTGTCTGCTGCGACTCGAGA50.163826998689384No Hit
ATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAA50.163826998689384No Hit
GTTCTCCACTGGAAAGGTTGTTTACGACAAACCTCCTTGTCAAAGTGTGTAAAAATAAAGGATTGCTCCAT50.163826998689384No Hit
CCTCTAACCTGTGCCACAGGTGACCACCAATAAAATCCTCTGCTGAGTAAAAAAAAAAAAAAAAAAAAAAA50.163826998689384No Hit
AGTTACCTCTTCATGTTTTAGACAGCAAACCCTATCCATTAAAGTACTTGTTAGAACAAAAAAAAAAAAAA50.163826998689384No Hit
TCTTTCATGCTTGCAAGTGAAATATGTTACAGAACATGCACTTGCCCTAATAAAGAATCAGTGAAATGGTC50.163826998689384No Hit
CTGCCACGTGCTCCCTGCCAGCTTCCCCCCTGAGGTTGTGTATCATATTATCTGTGTTAGCATGTAGTATT50.163826998689384No Hit
GCATTAATTAATTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
ACGGTCTGGCAGGGCAGCAGGAGGCTTTCTGGGCTCTGGACTCCACGGATGCGTGGGCAGAGGAATGTGGG40.1310615989515072No Hit
GGCTAGCTGATTTCTTAAAAATTCGTTGCATTGAAGGATATTTTGCATGTCTGTAACACCTGTCAATACTT40.1310615989515072No Hit
ATTACAAATTGGATGGAACTGTGTTTTTTTCTGCTTTGTTTTTTCAGTTTGCTGTTTCTGTAGCCATATTG40.1310615989515072No Hit
GGGGTCACAGTGAGCCGAGATTGCACTACTGCACTTCAACCTGGGTGACAGAGGGAGACTCCGTCTCAAAA40.1310615989515072No Hit
GCTAAGCATCTCCTTACAGTCACTAATATAGTAGATTTTAAAGACAAAATTTTTCTTTTCTTGATTTTTTT40.1310615989515072No Hit
GTGCATTATTCTGAGAATAAATTTCTGTTTCAAACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
ACTTGTAGTAGTTGACTTCTCAGCCACGTGGTGTGGGCCTTGCAAAATGATCAAGCCTTTCTTTCATTCCC40.1310615989515072No Hit
CTACTAAAAATAGAAAACTTAGCGGGGCGTGGTGGCGCCTGCCTGTAATCCCAGCTACTCGGGAGGCTGAG40.1310615989515072No Hit
GAGAAATAAAGATGCCTCAGTGTGGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
GAGCTGAGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAAAA40.1310615989515072No Hit
CCATCTTCATTCCTGCACCTTAAACCTGTACAGTCATTTTTCTACTGACTTAATAAACAGCCGAGCTGTCC40.1310615989515072No Hit
GTGTACAGAATGCTAAAATAATTAAAAAAAGACAAAATATACCTCTTCCTACAAGATTATTTAACTTAAGT40.1310615989515072No Hit
GGAGAGAATCACAGATGTGGAATATTTGTCATAAATAAATAATGAAAACCTAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
GAGGGAGGATTCGTCCCATTACAATAATGAAATAATGATATTCTAATTTTTTTAAATAAAATGTTAAGCCT40.1310615989515072No Hit
ATAAAGTTCTTTGCCTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
GGAAACCCAGAAACATTTTACGTTGCTTTTACTTCTGTAGTGTAGATTGCCCCGGCCCCTCTCTGAGCCCG40.1310615989515072No Hit
GGAAACCCAGAAACATTTTACGTTGCTTTTACTTCTGTAGTGTAGATTGCCCCGGCCCCTCTCTGAGCCCA40.1310615989515072No Hit
CCCTACACGACGCGGGGCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1310615989515072No Hit
GAGATATGCTAATTTAACTTACAAATGATTTTAATAAAATCTTGAGTTTGTATCACATGTTACTTATTGAC40.1310615989515072No Hit
GGTTTTCTGGCTGAAGCAATTTCCAGACTCTGGTAGGGGAAGCCAAGTAGTGCTTAGAGGTCTCCGTGAGT40.1310615989515072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)