FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53004
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7921.4942268508037129No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2850.5376952682816392No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA2500.47166251603652554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.46222926571579503No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT1470.277337559429477No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC1010.19055165647875633No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT1000.18866500641461023No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT950.1792317560938797No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA750.14149875481095767No Hit
CTACTAATATTTTGTATAAAGCAATTTTTTGTTCCATTACGTGACTTTTTGTTTTATTGTATATGTAATTT740.13961210474681154No Hit
CAATAAAGGGTAAAGTTGCTTCCCCAAACCACACTTTTAATCAAAACCTAGAATCATCTGCAGTCCTTGTT660.12451890423364274No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA630.11885895404120445No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC620.11697230397705834No Hit
GGGAATCTCCACTCCGGAGGAACTGGGCCTTGACAAAGTGTAAACCGCATGGATGGGCTTCCCCAAGGATT600.11319900384876612No Hit
ATGTAGTATGCTCCGTATAGTGATTGTAGCTGTTCCTCTGGATTCACCATCTGTTGAGTTGTAAATGTGAG570.10753905365632782No Hit
ATGGTGGATAGCGCAGAGGATCAGAAGATTCACCGGCCCATGGCTCCCAAGGAGGCCCCCAAGAAGCTGAT560.10565240359218173No Hit
AAACAATGATTTTAATATATCTCTTTCTTTTTCTTCCGACATTAGAAACAAAACAAAAAGAACTGTCCTTT550.10376575352803563No Hit
CTCTATAATAGAGTATATAGGTTTTGTACCTTTTTTACAGGAAGGTGACTTTCTGTAACAATGCGATGTAT550.10376575352803563No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTT540.10187910346388952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)