FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14535
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5133.5294117647058822No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1290.8875128998968008No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA970.6673546611627107No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC470.3233574131406949No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400.27519779841761266No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC390.26831785345717235No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT360.24767801857585142No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC320.22015823873409013No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC310.2132782937736498No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA300.20639834881320948No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT280.19263845889232886No Hit
GCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGT260.17887856897144824No Hit
ATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTT250.1719986240110079No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC250.1719986240110079No Hit
GAGTCATAGTGAACAATAAAGGCGAGATGAAAGGTTCTGCCATTACAGGACCAGTAGCAAAGGAGTGTGCA230.15823873409012726No Hit
TTATAACACAGTGTGGCAACGCACGAGCAAGTAACCATTAGAGTAACATTACTTTGTCCAGTAAATGCTTC230.15823873409012726No Hit
GTTTTCAACAAAAACAACAAAGAAAAAAGGGATGGACAAAAAGGCATTTATACAAATCTAGGGTGAGGAAT220.15135878912968698No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC220.15135878912968698No Hit
GGTTTTTCTTTTTTCCCACAGAGCTCGGTGGTGTTGATTCCATACAGTTTTTGTTCAGACAGGAAGGGATA220.15135878912968698No Hit
AATTTAATACACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATTTCATAAGACATTAAACAAAG210.14447884416924664No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA210.14447884416924664No Hit
CATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTC210.14447884416924664No Hit
GGGTAGCACTGGGAACAGGAGACCCACCTGAGGCTCAGCCCTAGCCCTCAGCCCACCTGGGGAGTTTACTA210.14447884416924664No Hit
AATTAATTGTAATCTGAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATG200.13759889920880633No Hit
CTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTA200.13759889920880633No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA200.13759889920880633No Hit
GGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATTAGTGTTCTACACCA200.13759889920880633No Hit
ATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATT200.13759889920880633No Hit
ACCTATAAGTCTAATGGCTTTCAAATGTAATTTCCATTTGCTAATGGTGATCTTGCCACATCTGGCACGGA200.13759889920880633No Hit
GCAATATGCCGTGTAGAATATTTGTCTTAAAATTCAAGGCCACAAAAACAATGTTTGGGGGAAAAAAAAGA190.130718954248366No Hit
ATCAAAGACAAAACCAGTTTGAAAAACCTTTTGTATTCTACAATACCTTTTATGAGTAAGAGTAAAACATA190.130718954248366No Hit
CCTTAGAGAGAAGTGGGGTGGCTTTTAGGATGGCAAGGGACTTCCTGTAACAACGCATCTCATATTTGGAA190.130718954248366No Hit
GATTATAAACAATGATGCTGGGTAATGTTTAAATGAGAACATTGGATATGGATGGTCAGATGAAAGATACC190.130718954248366No Hit
GCACATGCAGTGCTAGCATCTAAACTAATACAATAAGCAATTACTAAAGCTACAGTGACTTGAGGTTCAAT190.130718954248366No Hit
GTCAGGAGATTGAGACCATCCTGGCTAACATGGGGAAACCCCATCTCTACTAAAAATACAAAAAAATTAGC180.12383900928792571No Hit
CTAGATGGCAGCCGGCTCATAAAGGTTCATTTGGACAAAGCACAGCAGAACAATGTGGAACACAAGGTTGA180.12383900928792571No Hit
GAAAAGAAGCCATTGCTAACTACTTCAACTGACAGAAACCTTCACTTGAAAACAATGATTTTAATATATCT180.12383900928792571No Hit
CTCCACTATTGTCCTATGACCCTGCCAAATCCCCCTCTGTGAGAAACACCCAAGAATTATCAATAAAAAAT180.12383900928792571No Hit
GTATTAGAATGAGGCCGAAGATCACGGTGACCAGAGATTTCTAGGAGTCTCTAACCTTTCCACCCTATCCT180.12383900928792571No Hit
CTTTATCATCCACTCGTGATTTTTTAATTAAAGCGTTTTAATTCCTTTCTCTGTTCAGCTGTTGATGCTGA180.12383900928792571No Hit
GTGTACTGACTGAATACATTTAAAAGAAAATATATTTTGAAACCTGTTCTGCTATGAACAGAGATAACATA180.12383900928792571No Hit
GTGTATCTCGTGGCTTCACACCCGCCCAGTTCCAGGCGGCTCTGGATGAATATGAGGAGCTCAATGTCTGG180.12383900928792571No Hit
CTACCATAGATGTGACATTTTTGACCTTAATATCGTCTTTGAAAATGTTAAATTGAGAAACCTGTTAACTT180.12383900928792571No Hit
TGATGAAACCCTGTTTCTGCTAACAGTACAAAAATTAGCTGGGTGTGGTCCCAGGCGCCTGTAATCCCAGC180.12383900928792571No Hit
GTCGAGAGCTGATGGGCGGCATTGGGAAAACCATGATGCAGAGTGGCGGCACCTTTGGCACATTCATGGCC180.12383900928792571No Hit
CAGCAGTACAACAGTTTTGTGTCACTGTCTGTCTGAAATCTGCCTCCTGTGCCTCCCTTTTCTCCTCTCCC170.11695906432748539No Hit
ACCCAATATCTATTTTTTAACTGATTGTATAACTCTAAGATCTGATGAAGTATATTTTTTATTGCCATTTT170.11695906432748539No Hit
GCTTCATCTGTCCTGCATCGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCAGGA170.11695906432748539No Hit
GTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCC170.11695906432748539No Hit
TGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACTACTTCAGAATCCAGCAGCTCTC170.11695906432748539No Hit
CTAATTGCGGGCCTCACCATTGCATGCAATGACTATTTTGTAGTACACATGAAGCAGAAGGGAAAGAAGTA170.11695906432748539No Hit
TGATTATACAAAGCCAAATATGCTCATTATATAAAATTTAGAAGCAAAAAGAAGGAAATAAAAATTTTCCA170.11695906432748539No Hit
TGTTGTAAGAATCAAATGAAATAATGTATGTGAAAGCACCTTGTAAACTGTAACCTATCAATGTAAAATGT170.11695906432748539No Hit
GTAGACACAGCTCCTATATTGTTTTACAAAAAAATAAAAGCATGTCTTTCAACATGCATCCAAAACAGTGT170.11695906432748539No Hit
GAAAAATGCTGACAAGCACTAAAATCACTGTAAATTCCTTGGAGAGTTCTCAGAATTGCAGAAGTTCAGAA170.11695906432748539No Hit
ACATTCACATGGCAATATTAGACAGTTAAGCACCCAATACCCATAGTTGACAAAATGTCCCACTGACCAGC170.11695906432748539No Hit
GCCATAAACCCAACTTAAAATAAGATGGGTATATTTCTACTCTCTTGGTTTACATCCCATGCTGGCAAGCT170.11695906432748539No Hit
AAGGAAACTAATTGGACCATTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTTAT170.11695906432748539No Hit
ATTAAAGACTAGAAGGGCACAACTTTTTTTTTAATTACCATAGAGAAGATACATTAAAAAAAATCTTCTGA170.11695906432748539No Hit
CTTACACAGTTAGTCATGGAGAGTAACAGGCCTGCTGGTGAAACAGGTCACCCAAAATGGAGATGGCATCA170.11695906432748539No Hit
GTTAATAATCATGTTATGTTAATTTTAACATGATTGCTGACTTGGATAATTCATTATTACCAGCAGTTATG160.11007911936704506No Hit
CTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC160.11007911936704506No Hit
CCTTAATATCGTCTTTGAAAATGTTAAATTGAGAAACCTGTTAACTTACATTTTATGGATTGGCACATTGT160.11007911936704506No Hit
ATTCACCTGAATAGTCAGGGAACTTTCACCTATTTTATTTAGGTTTTCTTTTTCTTTTTTTCTTTTTTTTT160.11007911936704506No Hit
GGTTGGAGTGCAGATGGCACAACCTCTGCCTCCTGGGTTCAAGTGATTCTTCTGTCTCAGTCTCCTGAGTA160.11007911936704506No Hit
GCTTGTCTATACAGTATCATATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACATAAGC160.11007911936704506No Hit
TTCTATTCTTGTTTGTTGGAAGCTAAAAGCTTGATAAAAGGAATTGGTGTCTTTTGATACAATCAAATTTT160.11007911936704506No Hit
GCTAATGAGGAAAATGGGGAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGAGGA160.11007911936704506No Hit
GGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAGG160.11007911936704506No Hit
GATCAGGACACTTAGCAAATGTAAAAATAAAATCTAACTCTCATTTGACAAGCAGAGAAAGAAAAGTTAAA160.11007911936704506No Hit
AGCCTATACAACTTGTAGTTCTACTTAATCACTGACTGGTACAGCTAACATGAGATAAGTGAAAAGTTCCT160.11007911936704506No Hit
ATATGAAACAATTTAAAAACCTTTAAAGGCGTATTGAGAAAAACCAGGCTTTTTTAAAAAACACGTTTGTT160.11007911936704506No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA160.11007911936704506No Hit
CTGCACATACTATACACTGAAAATTATTTAAAATGCTGAAAGAAATCAAAGAGCTGAATAAACTGAGATAT160.11007911936704506No Hit
GTATGTAGCATTTTGAGAGCGACTTCTTTTGCTATGAGTAATACATTTGAGATTCATCCATATTTCTCAGT160.11007911936704506No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT160.11007911936704506No Hit
GGTCCCGGTCACCATGGGGTTCTGGGTCCGCGTGATTGGACCTCTGTAAAGTGCAGACGCCGGATCCATGA150.10319917440660474No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT150.10319917440660474No Hit
GGTTTTTCCCTTTCCTCTATTTTGTAAATATAAGATGAGTATTATTTAATGATGTATTTGCATAAGTAATG150.10319917440660474No Hit
CACTATATGTGACTGTGACTTTCAAGGACATTTGTTTGCCATTTGCTGATTTTTTTGGGAAGTTAATTTCT150.10319917440660474No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT150.10319917440660474No Hit
CCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTAACACTACGAGAGACT150.10319917440660474No Hit
ATAAAGTGAGCATGTTGGTCCCATTTATAAATATATGTATGATTTATAAGCTTTTTTAAAACAAAGCTCAA150.10319917440660474No Hit
ACACATTATTCTCACACATCCGGGGATAGGTTTGATACACAAGATCCTTAAAGTCATTTTACATATTCTGA150.10319917440660474No Hit
ATATAGACCAATGGAGCAGAACAGAGCCCTCAGAAATAATACCACACATCTACAACCATCTGATCTTTGAC150.10319917440660474No Hit
ATTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTA150.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)