FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41525.01506931886679No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT291.7480409885473176No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG271.62748643761302No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA251.5069318866787222No Hit
CTTGCTGGACAGCTATCTTCCACATACTTCCCAGTTTACATTTGGTCTTAATGATCTTGAATAGATCCTCT191.1452682338758287No Hit
GATCCTGGGGACTGCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACA150.9041591320072333No Hit
GGATGTCAGCTGCCAATTGTGTTTTCCTGCAGCAATTCCATAAACACATCCTGGTGTCATCACAGCCAAGG130.7836045810729355No Hit
CTGGATAATCAGTGTATGTTGCACCAGAATCAGCATTTTTTTTTTAACTGCAAAAAATGATAGTCTCATCT120.7233273056057866No Hit
CTTTCCCTACACGACGCGGGTGGGCAACAAGAGTGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAA110.6630500301386377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.6027727546714888No Hit
GATCACGAGGTCAGGAGTTCGAGACCAGTCTGCCCAACACAGTGAAATGCTATCTCTACTAAAAATACAAA100.6027727546714888No Hit
CTCCGTTTTCTAGCAAGTATTAAGGGAGCTGTGTCTGAAATGGCACTGTCTTGTCAGTCATTTCTGTTTAC90.5424954792043399No Hit
GTTCCCCGGTACTTCCTCCTTTATTGTAGCACTCAGCTCCCCAGCCAATCTGTACATCCCTCAGAGGCAGC90.5424954792043399No Hit
CCATTATCCTGTGGAATCTGATATGTCTGGTAGCATGTCATTGATGGGACATGAAGACATCTTTGGAAATG90.5424954792043399No Hit
CCCATTGAGCTGTGTATGCATTTTTGTTTATTTCAATAAAATATATTTGTATTATTTGTCCTTCATACTAT90.5424954792043399No Hit
GGGCATTCTCTGTACTAGTTTGTCGTGGTTATAAAACAATTAGCAGAATAGCCTACATTTGTATTTATTTT80.48221820373719104No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA80.48221820373719104No Hit
ACAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGG80.48221820373719104No Hit
CATACTTGGTTGTAATATGTGATGGTTAACCTGTAGCTTATAAATTTACTTATTATTCTTTTACTCATTTA80.48221820373719104No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGC70.42194092827004215No Hit
GAATCTGAAGAATGAAGACATTGAGAAAACTATGTAGCAAGATCCACTTCTGTGATTAAGTAAATGGATGT70.42194092827004215No Hit
GTTTAAGAACTAAATCTTAATTTTTCCTTATAAGAAAGTCAATACATACTTAAAAAATAACCCACAAAAAA70.42194092827004215No Hit
GCTTTTAATGTTGCGCCTCTTCAGGTTCTTAAGGGATTCTCCGTTTTGGTTCCATTTTGTACACGTTTGGA70.42194092827004215No Hit
CCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGTTCCCTTTGGGTTCCAT70.42194092827004215No Hit
GGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGGCCCTGTAGTCCGCCACAGGGCTCTGAGCTG70.42194092827004215No Hit
CAGACTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCA60.3616636528028933No Hit
GCCTCACCGCTCCCAGCAACTGAAAGGACAACACTATCTGGAGCCACCCACTGAAAGGGCTGCAGGCATGG60.3616636528028933No Hit
CATCAACGAGGGGAAGCTGTGGAAACACATCAAGCACAAGTATGAGAACAAGTAGTTCCTTGGAGGCCCCC60.3616636528028933No Hit
GGGGAAGAGAACGTAAGAATGAGGTGAGGGGAGAGAATGAAGGTGAAGATTAATATAGATTAATATCAAAA60.3616636528028933No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA50.3013863773357444No Hit
AAACAAACAAAGGGCTTAGACACCGGCTAGTCGGCTGCGGGAGGCAGCAAGAGAAAGGCCGAGGGGCCTGG50.3013863773357444No Hit
GAGGGGGTCAGAGCAGGTGATATCCATGTTTCTTCCCTTTCTGATATTGTTGTCTGTGGCATATTCTTTGT50.3013863773357444No Hit
GGTGCTTCTCTAGGAGGGAAACAGGGAGACCTGGGGTCCTGTGGATAACTGAGTGGGGAGTCTGCCAGGGG50.3013863773357444No Hit
GCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAG50.3013863773357444No Hit
GTCGGAAGAAATTCAAAAGAAAAGAACCCGCCGAGCAGTCAAATTCCAGAGGGCCATTACTGGTGCATCTC50.3013863773357444No Hit
CAAAACTTTCTGAGTGCCAGTATGACGCTCAAAGGAAATTCTCATTAGAGCATTTTACAGTTCTGATTTCC50.3013863773357444No Hit
CCCTCATCACCCACACTCCCTATTTTGGGCCCTCACTTCCTTGGGGACCTGGCTTCATTCTGCTCTCTCTT50.3013863773357444No Hit
GGACAATTGCTTGAACCAGGGAGTCGGGTGTTGCAGTGAGCCGAGATCGTGCCGCAGCACACCAGCCTAGC50.3013863773357444No Hit
GGAGCCGTGAGCGTGGGGAACCCCACAGAGCCCAAAGGACTTTTTCAGTATTCGAAATAAACAAAACAAAA50.3013863773357444No Hit
GCTACTGGGAAGGCTGAGGTGGGAGGATCACTTGAGCCCTGGAGGCAAAGGTTGCAGTGAGCTGAAATCGT50.3013863773357444No Hit
GGAGGGGGACTTTAGAGAGGGATGAAAATGAGCCCTGGGAGGGAGGAAGGGACGAGGAGGGGTGGCTGCAT40.24110910186859552No Hit
CCTTCAGCTGTCTTGCTTATGTACCGTATGTAAATTTATTCTTTTTAAAAATCACTTTTATTTGATTTTGA40.24110910186859552No Hit
ACCACCACCTCAGGGAGTTAAGCGAAAAGCTGGACAAATTGACCCAGAGCCTGTGATGCCTCCTCAGCAGG40.24110910186859552No Hit
GTTGAGACGGATTCTCTATGTTGCCCAGGCTGGTCTCAGGCTCCTGGGCTCAGATGGTCCTCCTGCCTCAG40.24110910186859552No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.24110910186859552No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.24110910186859552No Hit
CAATCCTAGGCACCGGCAAATACAGACAATAGACCAAAGTCCCTGCCCTCGAGGAGCTTTCATTCTGATGG40.24110910186859552No Hit
AGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGACTAATCACCAAAAAGCAACCAACTTAGC40.24110910186859552No Hit
CCATCGTTAAAATTATTTATGTGTAACATTCAAATGTGTGCATTAAATATGCTTCCACAGTAAAATCTGAA40.24110910186859552No Hit
GGCTACTGCTAAGGCCTGAGAGTTTTTGCAGAAATGGGGCAGAGGGACACCCTTTGGGCGTGGCTTCCTGG30.18083182640144665No Hit
GCTCAAACTGTTTTCTTGATCTAAAAAATGGAGGTAATCTAGGCTGGGCACGGTGGCTCACGCCTGTAATC30.18083182640144665No Hit
GTGGTAGAAAGGATTTGGAAGAGCAAATTGCATTGTGTGCACAGATAATTGTCCATCCTGGAAAATATCCA30.18083182640144665No Hit
ATATTCCTTTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
CTCGTTGGGTCACGCCTTTCCCATTCCTGAAGAATAAGCGGAGTGCTTCCTGCAGCCGAAGACTCCATGCC30.18083182640144665No Hit
GGAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
TAATAAGAATAAGTAATAATAAAGAAATTTCTAACATTCCATGTCAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
ACCCTGTCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
GGGTGACAGAGCAAGAGTTGGTTTCAAAAAAAAAAAAATAAAAGAAATATTGGCCAAGGATTTTCCAAAGT30.18083182640144665No Hit
GCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAA30.18083182640144665No Hit
GGGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
GGAATGAGATGTCCCTGAGCTGTACTTTTCTATTATTATAAGGCCTTTAGGCATCAGTGCATCTGGGTTAT30.18083182640144665No Hit
TGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
GTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
CCTATGAATTCATGGCATAATAGGTGTTAAAAAAAAAAATAAAGGACCTCTGGGCTACAAAAAAAAAAAAA30.18083182640144665No Hit
GGCCAGTGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
GGTGCAGCGCTTCACAATGCTAAAGCCTTAGCCCTCCTCCAAGAGCTGAGACCTCTCAGGGCCTGAATCTT30.18083182640144665No Hit
TAATAAGAATAAGTAATAATAAAGAAATTTCTAACATTCCATATCAAAAAAAAAAAAAAAAAAAAAAAAAA30.18083182640144665No Hit
GTGTTTACAAGGGAGGAAGGACCTTTCCTCACCTCCCTTTTGACAGAGATTAGAAGTACTTCTTTAAGAAA30.18083182640144665No Hit
GCTTGTCAGGCTGAGGCATGAGAATCATTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGTGCC30.18083182640144665No Hit
GTTTTTTTGTTGGATATGGAGTTACTCTTTCCATGGCTTACAGGTTGCTGAAAAGTAAATTGTACCTGTAA30.18083182640144665No Hit
GATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCATCTCTACTAAAAATACAAA20.12055455093429776No Hit
CAGTTAGCACAGTATAACTTCATTTTAGTTGTTGGTGAAAAAGAGAAAATCAGTGGCACTGTTAATATCCG20.12055455093429776No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGTGCCCGTATTTACCCTATAA20.12055455093429776No Hit
GCTTTCTTATGCCAATCAGGCCCCAATAAAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTG20.12055455093429776No Hit
GCTCTTGCGGGATGGCCTGTTTCGTTCCCCAGTGAGAGGGAGCCGGTCCTTGCTGCACCTGTGCCGTCCCC20.12055455093429776No Hit
GGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATC20.12055455093429776No Hit
CCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GGATTTTTAACATTAGAAGTAGGTACAGAATACTCAAAATTATTCTTCTATCATTGAAACTCTTTGTTGCA20.12055455093429776No Hit
GAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCAAGCCCCAGCGCCTATATTAAGGCACATTTGAAT20.12055455093429776No Hit
GCATTTGTTTTTCCTGGGCAGTCCTGTCAGCCAGTTAATCCACCAGCTCTTAGGAAGTAAATACAGATTTT20.12055455093429776No Hit
CCCTACACGACGCGGGCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTATAATCTCAGCTACTTGGGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCGGTG20.12055455093429776No Hit
GTCTATATATGTATTATCAAATATGTAAGAATACAGGCACCACATACTGATGACAATAATCTATACTTTGA20.12055455093429776No Hit
GTGCAGCTCTCCACTGGAAAGGAACTCTCCACCCCTCCCATCCTGATAAAACAAACAAGATTTACATTTAC20.12055455093429776No Hit
GATACTATATACGTTTTGTATATCATTAAATCTTTAAAAGAAATGAAATAAATTTATTGTTTACAGAAAAA20.12055455093429776No Hit
GTCTTGGAGCTGTTGTACATTTAAGAATAAACTTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GTCATTTGACAGTAGTAGAACCTATTCTAAAACCAAAAACTATTAATATCACATCCTTTAACCCTAATCTA20.12055455093429776No Hit
ATTTTGCTCTCATGTGTAATGTTTTCTATTTAAATCTTTCTTAAATATACCAGCTGTTCTCTTTCCCTGAA20.12055455093429776No Hit
GGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GAGAATACCTGTTTAGTGTTGCACTTTAGACTAGTGTCTATTTTATTAATACAACTATACCACAAATTCTC20.12055455093429776No Hit
TCCCTACACGACGCGGGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GCACTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATACCCATCATCCTAAAATTAATTCCCCTAAAAATCT20.12055455093429776No Hit
GTTGGAATCGTTTCTTTTGTTTGTCTGATTTATGGTTTTTTTAAGTATAAACAAAAGTTTTTTTATTAGCA20.12055455093429776No Hit
GTAATAAAGAGAGAAATTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTT20.12055455093429776No Hit
GCTCCTCCCTGGTCACCCTGAGGGAGCTCAGGGCCCGGCTGGTAGCTGGGTTGCTCTGCTTCTGTCCCCGA20.12055455093429776No Hit
TTTTTATTACATGCGGTTATTTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CATGAGAACAAAACCTCTTCTGTATTTTTTTTTTCCATTAGTAAAACACAAGACTTCAGATTCAGCCGAAT20.12055455093429776No Hit
GGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGGCCCTGTAGTCCGCCACAGGGCTCTGCGCTG20.12055455093429776No Hit
GGGACATTCTCTACACTCAGATGACTCTACCAGTGGCCTTTTAACCAAGAAGTAGTACTTGATTGGTCATT20.12055455093429776No Hit
GAGCGGGAGAAAGAACTGGCAAAAGTCACTATCAAGAAGGAAGATCTGGAGCTAATAATGACTGAGATGGA20.12055455093429776No Hit
GTGTGTCCCAGGCCAATAAAATCTACCTGCCACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GAGTATGTTGGACTCTGCCATAGACTGACCCTCTTATCTAACTACTACAATTTATCTATAATACCCTAACA20.12055455093429776No Hit
CAATCCTAGGCACCGGCAAATACAGACAATAGACCAAAGTCCCTGCCCTCGAGGAGCTTTCATTCTGATGA20.12055455093429776No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GGCCAAGCAGGGCAACCCCACACCCTTGACATAAAAGCATCTTGAAGCTTTTAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GTGGAATCTATCAGCATTGTTGAAATGCTTAAGACTGCTGCTGATAATTTTGTAATATAAGTTTTGAAATC20.12055455093429776No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
TCCCTACACGACGCGGGAGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
CCTTCCCAAATCCTATAAAATGGCCCCATCCCTATCTCCCTTCGCTGACTGTCTTTTCGGACTCAGCCCGC20.12055455093429776No Hit
ATTATTGATGTTTTCATAGAGAATATAAAAATAAAGCACTTATAGAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
TGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
GAACCGCTCCTACAGCAAGCTGCTGTGCGGCCTGCTGGCCGAGCGCCTGCGCATCAGCCCGGACAGGGTCT20.12055455093429776No Hit
ATGCTCTGAAATCTCTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT20.12055455093429776No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCC20.12055455093429776No Hit
AAAAAAGACAGTATATTTTGTACTTTATAAAATATTAATTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
TCCCTACACGACGCGGGAGGGGGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit
AAAAAAGACAGTATATTTTGTACTTTGTAAAGTGTTAATTAAAATGAAAAAAAAAAAAAAAAAAAAAAAAA20.12055455093429776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)