FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53528.33686440677966No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG382.0127118644067794No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT281.4830508474576272No Hit
GTTGGTCAACTCTGATACAAAGTATATATACAGTTCAGTATTGTCTCTGTTCATTTTGTTTTTATTTCATT191.0063559322033897No Hit
GTGTTGGAATTACAGGCGTGAGCCACCGCGCCTGGTCTATATGTGATTTCAAATTAGGTGGCCTTATAAAT160.847457627118644No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA140.7415254237288136No Hit
GTATGTTAAAGATGTTAGATGATAAAAGATCTGTAAAGGAAATTTGTTTTAATTCAAAAGAAAATTCTAAC130.6885593220338982No Hit
CAGCTGAGGAAAGGGAGACAAGGTATTTTATTTCTGACTGATTTTAGAAAAAACTTGTGTACATGTGTTTG120.6355932203389831No Hit
CATTTATAGATTAATTCAGAAAAACCATGTGATTGTTTCAATAGATGCAGATAAATGATTAAATAATTTCC100.5296610169491525No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG100.5296610169491525No Hit
GGGTGTGGTGTCTGGCGCCTGTAATCCCAGCTACTCCGGAAGCTGAGGCAGAGAATTGCTTGAACTGGGGA100.5296610169491525No Hit
GGCCTTCGCCGCCGCCTCGCGGGAGGTGCTGGCGGTGGAAGCAGAGCTGGGCGGGCCTCGCAGGCAGCCGC90.4766949152542373No Hit
AGCCCAGAAGGTTCCTGCCCAGAAAGCCACAGGCCAGAAAGCAGCGCCTGCTCCAAAAGCTCAGAAGGGTC90.4766949152542373No Hit
GCTTTATAATAAACTGTTTAACGTGAGGTTTCAGTAGCTCCTTGGTTTTGCCTCTTTAAATTATGACGTGC90.4766949152542373No Hit
CATTCGAGCCCAGGAGTTCAAGACAAGCCTGGGCAACATAGAGAGACTCTGTGTCTACCAAAGAATTAAAA90.4766949152542373No Hit
GCTGTGATTGCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTG80.423728813559322No Hit
GCTCAGTGCTTGGGCTCCACGGGCAGGGGTCAGAGCACTCCCTAATTTATGTGCTATATAAATATGTCAGA80.423728813559322No Hit
AGGAAATGGACTGCCTCTCTTGAGCACTTCCCTGGAGCTACCACGTATCTAGCTAATATGTATGTGTCAGT80.423728813559322No Hit
CATTTAGAGAATGTCCTGGCGTCGGTTTTTAGCATATGTGGTATTTAAACAGAGCTAGAATGTGATGTCTG80.423728813559322No Hit
GTTAATAAATTACCAATCAGGCACATTTTATAATGTTTGTCTCTGTAAAGGTAATATTAGCAGTTAAAGAA80.423728813559322No Hit
GGGAGCGAGAAAAAGAACGGGAGCGTGAGAAAGAACTTGAAAAAGAACAAGAACAGGAGCGAGAGAAGGAG80.423728813559322No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC70.3707627118644068No Hit
GCCCGACCCTCACTACCCCTGAGATATTAGTTCCCAGGCCTGTTTTCCCACAGGATTGTGGGCTCTCTGCT70.3707627118644068No Hit
GCTCTGGAGAAGATTGACCTTAAGTTCATTGACACCACCTCCAAGTTTGGCCATGGCCGCTTCCAGACCAT70.3707627118644068No Hit
GGTATCTGCAAAAGCAATTAGACGCTACCACTGATGAAAAGAAGGAAACAGTTACTCAACTCCAAAATATC60.31779661016949157No Hit
GATGTATGTAGACCTAAAACTACACCAATAGGCTGATTCAATCAAGATCCGTGCTCGCAGTGGGCTGATTC60.31779661016949157No Hit
GGGAAGATCCTGCGGCTGGGGCCGTCTGCCTACACGCTCCAGAAGGGCCAGCGCAGAGCTGGGATGGCCAC60.31779661016949157No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA60.31779661016949157No Hit
GTCAATTTGTGTTGCTGTGTTGGTTGTTGATGAAAATAATAAAATGATTGATTACAAAAAAAAAAAAAAAA60.31779661016949157No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC60.31779661016949157No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.31779661016949157No Hit
GATGGATCAATAATAATGAGGAAAGCATGATATGTATATTGCTGAGTTGAAAGCACTTATTGGAAAATATT50.26483050847457623No Hit
GCACTTTGGGAGGCCAAGGTGGGTGGATCACTTGAGTTCAAGACCAGACTGGAGGACATAGCAAGACCTCG50.26483050847457623No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCA50.26483050847457623No Hit
GCTCCTGCTGCAGATAACTAGCCAAACCCATCCCTTTATTTCCGCCCATCCCTTTGTTTCCCATAAGGAAT50.26483050847457623No Hit
GCCTCCCAAAGTGCTGAGATTACAGGTGTGACCTATCACATTCAGCAAAATATCTATACTTCCCAAAGCAA50.26483050847457623No Hit
CATCTAGTATGTGCCAAGAGCCCTAAAAATGGTTATCTTGCTAATATTACTTCTGGGACTTTATTCTATGA50.26483050847457623No Hit
GGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAA50.26483050847457623No Hit
GAGAGAGACCTCATCTTTCATGCTTGCAAGTGAAATATGTTACAGAACATGCACTTGCCCTAATAAAAAAT50.26483050847457623No Hit
TCCCTATCTCTTTCTTAGGAATGTTACCAAGCATATTTTGAAAAGTCCAAGTATTTTGATAAAGTATAAGA50.26483050847457623No Hit
GTGATCCGAGATCACACCATTGCACCCCAGGGGCCTAGACAACAAGAGCGAAACTTCGTCTCAAAAAAAAA50.26483050847457623No Hit
AATAAAGACCTGAGAACCTTCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.26483050847457623No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.26483050847457623No Hit
GGGATGAGCTAGCTGTTAGAGCAAGGGTTTTGGTGAGAGATAATATTGATTGAAGGGATTTTAAAGGAAAT40.211864406779661No Hit
GCAGCTACTGGATCAAAATCAAGAAATCCGGGCATCACAGAGAAGTTGGGTTTAGGACAGGTGCTGTTCCG40.211864406779661No Hit
GCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTAC40.211864406779661No Hit
GCTTATGGCACAATTTGCCTCAAAATCCATTCCAAGTTGTATATTTGTTTTCCAATAAAAAAATTACAATT40.211864406779661No Hit
GTGTCCACAGCCACAAAAAGAAGAAGATCCGCACGTCACCCACCTTCCGGCGGCCGAAGACACTGCGACTC40.211864406779661No Hit
GAGGTTATGGTGAGCCAAGACTGTGCCACTGAGCTCCAGCCTGGATGAAAGAGGGAGACTCCTCTTCAAAA40.211864406779661No Hit
ACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAA40.211864406779661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.211864406779661No Hit
TTAGGAAGCGCAGAGTTTCACTTTGGTTTTGTTCATGGATGTTTAGAATGTTTAAGTAGGAAAAGAAGCCA40.211864406779661No Hit
GTGACACCCCTGCCCATTTTCTGTCTTTAATTAACCAAGGTGTTAGGTGTGACTGTCACAACTGTTATGTT40.211864406779661No Hit
CCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.211864406779661No Hit
CCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
GTTAAATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
TCCCTACACGACGCGGGGGGAATTGTAAATAAATATATCAGTTTTTTTCCCCAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
GGATTGCTTGAGCCTAGGAGTTTGAGACCAGCCTGGGCAACAGAGTAAGACCCCCATCTCTATAAAGAAAA30.15889830508474578No Hit
GCAGAGCAGCTTGCCGAGGACCCCGCGTGAAGAAAGCCAGCGAGGGGGGCTTCTGCTCCCTGAGACTCTGG30.15889830508474578No Hit
TTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGA30.15889830508474578No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
CCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTAGGGAGCCGCACCTTGTCATGTACC30.15889830508474578No Hit
GGCTTCTACAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
GACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGT30.15889830508474578No Hit
CTTTCCCAGCTCCCCACCCTCCTTGCCTTTGGGTGCGCGACAAACAATCGCTCCGGGCTCAGGGCTGCGCG30.15889830508474578No Hit
CCTCTCTCCCCCTGGGCCACTCCCGGGGGTGAGGGGGTTACCCCTTCCCAGTGTTTTTTATTCCTGTGGGG30.15889830508474578No Hit
CCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
CATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
GCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCC30.15889830508474578No Hit
CGACGCGGGGCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15889830508474578No Hit
GGGACCCTGGGACGAGGCTGCAGAAGCTCTCCCTCCCTACTCCCTGGGAGCCACGTGCTGGCCATGTGGCC30.15889830508474578No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTC30.15889830508474578No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA20.1059322033898305No Hit
GGCTCATATGATTTCACAGAGATTCTACCCAACCTCCAGAAAGCATATACACCCAATGCTGTTTGAATTTT20.1059322033898305No Hit
GTTTCTATCCAAAAGAGCAATAAAAAGTTTTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GGAGAGATCGGGAAGGTGTCCATTTGGAGTCATCCACGCAGAGATGTGTGAAGGCTGCTCAATGATTTTGA20.1059322033898305No Hit
GACATGTTGGTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GTGCCAAGATTGTGCCACTGCACTCCAGCCTAGGCAACACAGCAAGACTCCATCTCAAAAAAAAAAAAAAA20.1059322033898305No Hit
ACATAGTAAGACCCTATCTCTACCCCCCTAATAAATTAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
CTTCCGATCTCCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
AAGTTATTTTCTCATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
TTCCCTACACGACGCGGGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA20.1059322033898305No Hit
ATGCCAATGGCAACAGCTTTGCCACTCGACTTTCCAACATTTTTGTTATTGGCAAGGGCAACAAACCATGG20.1059322033898305No Hit
CATCTGGACAAATGCCACCACAGCAGGTGGGGTGGCAAAGCTACCTGGAATGGATTTGTGTGCTGATTTTT20.1059322033898305No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTAAAAACAAAATACTCAAATA20.1059322033898305No Hit
GAATTACAAAACTCAGAGAAATGTGTCATCAGGAGAACATCATAACCCATGAAGGATAAAAGCCCCAAATG20.1059322033898305No Hit
TGTTAAACTCTAATTGTGAAGGCAGAATCTCTGCTAATTTTTCAGATTAAAATTCTCTTTGAAAAAATACA20.1059322033898305No Hit
GGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
CTATTGAAAGTGCAGTGAATGTGGTGGACTGTTTCTTCTATGATGGAATAAAGGCCATTTTGCAACTGGGA20.1059322033898305No Hit
GTGTTGTGGTGGGGCTCCCAGGAGGACTGGCCCAGAGAGCCCTAAAATAACAAAAATCCTAAACTAAACTA20.1059322033898305No Hit
GGGACTACAGGCGTGAGCCACTGCGCCCGGCCTCCCCAAGGAGCACTTTTACAGCCAAACCCACCTCTCTC20.1059322033898305No Hit
CGGGAGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA20.1059322033898305No Hit
ATCTACTTGGATCCCATGCCTGAAATTTGGAAGCATATGTACAAAAATCATTTTTACGTTTTATTTTTAAT20.1059322033898305No Hit
GCCGTGTAGTCATCAAGACAGTAAATCCCAGGGCTTGGTTAAGTGCTGTGTGATAACTTGTTTGGATGAGA20.1059322033898305No Hit
CTCTTTCCCTACACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GAACCAAGAGTGTGACTGCCAAAACTTAGTGTGGCATCAGCACCAACAGCACAGTTCTTCATATCCACGCC20.1059322033898305No Hit
CCATGGTCTTTCTGGGCCTTACTGTGCTGGTATAATTAAAATAAAATATATTTTATTCTAAAAAAAAAAAA20.1059322033898305No Hit
TAGTATGATCCTGGCTAGAATGATAATTAAAAGTATTTAATTTGAAGCACCAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
TCTAGGTGCAGGGCCCTCGTCCGGGTGTGCCCCAAATAAACTCAGGAACGCCCCGGCAAAAAAAAAAAAAA20.1059322033898305No Hit
GGGGGGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
CCCCAGTAGAGCTGGGACTACAGGTATGCGCCACCACACCTGGCTAATTTTTTTAATTTTAATTTTTGTAG20.1059322033898305No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
ATGTAATACATATATTTACAAATAAAATGCCTCATAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GGTCCCAGCACCGATGGCATTCCCTTTGCCCTGAGTCTGCAGCGGGTCCCTTTTGTGCTTCCTTCCCCTCA20.1059322033898305No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
GAACTAGGGCCTTCTGCTCGCCCACCTCCCACCCCTACCTAAACAAACCCAAACTTAAAAACTCTAAACTA20.1059322033898305No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
ACCACATGCTGTGGCTTGAGCAACCAGAGAATATCGCCACTTGTATTGCTCTCCGGCCCTACCCCAAGGAA20.1059322033898305No Hit
TCCCTACACGACGCGGGTGACAATAAAGAGATTGGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
CTTCCCACCCCTCGGCAGTGGCTGTGCAATGTTTTAAGTTCACAAGTTCCTGCTCCTCCCCACACTGAGCG20.1059322033898305No Hit
GGGACTCTAGAGAAGATGCGAAAAGATCAAGAGGAAAGAGAACGTAACAACCAGAACCCCTGGACATTTGA20.1059322033898305No Hit
CCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTAGGGAGCCGCACCTTGTCATATACC20.1059322033898305No Hit
GGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCTAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit
ACGCGGGACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1059322033898305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)