FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002875991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002875991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48328.146853146853147No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG331.9230769230769231No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT251.4568764568764567No Hit
GATAGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAA231.3403263403263403No Hit
GCAGGAGGTCCTGCCCCCTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGA120.6993006993006993No Hit
GGTGGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTGCTTAGCAAGTTCCGGATAGA120.6993006993006993No Hit
GGAAGACACACAGTTTATTTGTAAAGTAGCTCTGTGAGAGCCACCATGCCCTCTGCCCTTGCCTCTTGGGA110.641025641025641No Hit
GCCTTGAACCCTTGCAAAGGGGCAGGACAAGGGGACCCCTCTCACTCCTGCTGCTGCCCATGCTCTGCCCT110.641025641025641No Hit
AGTTTCTAAATCTCTCTTTGTATAAGTTCCAAGAATTATAAAAATAGCACTATTTTCTGATCAAACAGTTA90.5244755244755245No Hit
GATTTTAATTATTGGAATGCACAATTTTTTTAATATGCAAATAAAAAGTTTAAAAACTTAAAAAAAAAAAA90.5244755244755245No Hit
GGTGTGGGCCACCGATTGGCGGCTGATCCTGGACTGGGTACCTTACATCAATGGCAAGTTTAAGAAGGATA90.5244755244755245No Hit
GTATAACTTCATTTTAGTTGTTGGTGAAAAAGAGAAAATCAGTGGCACTGTTAATATCCGCACAAGAGACA90.5244755244755245No Hit
ATGCCCATGTTGGTCCTCTGCCCTGGACCTGTGACATTCTGGACTATTTCTGTGTTTATTTGTGGCCGAGT80.4662004662004662No Hit
CAGCTTAATGAGAGTAATCTGCAGACAATTGATAATAACATTTTGAAAATTGGAAAGATGGTATACTGTTT80.4662004662004662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.4662004662004662No Hit
GCTTGTTTCCAGATATCACCCTCAGATAGCTCTAACTAGTCAGTCATTATTTACCTTATTAAATAATCATG80.4662004662004662No Hit
GGTCCTGGGAGTGGGAAGCCTCTTCCTTGTGTCTTTCGCTCCACTTTTAGCTCATCGCACCAATATTGCAG80.4662004662004662No Hit
GGGGCATCCACCTTCCTGCCCAGGGGACGTGGCGCTGTCGGTGTTTGGAGGCTGCTGCCCCCTGGCTTTGG80.4662004662004662No Hit
GGCAATCGAGACCATCCTGGCTAACACAGTGAAACCCTGTCTCTACTAAAAAAATACAAAAAATTAGCCGG70.4079254079254079No Hit
GTATCACTATAATTTTTCCTGTTCTTGAACTTCATGTAAATAGAAACAGTATGTATATTCTTTTGTACTTG70.4079254079254079No Hit
GCAAGGCTCTGCAGCCAATGCAGAAAATGGGTCAGCTCCTTTGAGAACCCCTCCCCACCTACCCCTTCCTT70.4079254079254079No Hit
GGGTGCCCTGAGTGGTGGTAAAGTGGAGCAATCCCTTCACGCTCCTTGGCCATGTTCTGAGCGGCCAGCTT70.4079254079254079No Hit
GTGTATACACATAAAGCAGGTCTTTTATCCAGTGAACAGGATGTTTTGCTTTAGCAGCAGTGACATAAAAT70.4079254079254079No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4079254079254079No Hit
CAGTTATTTGATTTTTTAAAAAATATTATTCCAAAAGAAAACCATTTTAGGTCATCTCCCCCAACTCTGTT70.4079254079254079No Hit
CAGATGGAGCAGCTCAACGGGATCCTGCTGCACCTTGAGTCAGAGCTGGCACAGACCCGGGCAGAGGGACA70.4079254079254079No Hit
CTTCAGAAAATCCATTCCCCAGTCATGAAAATGTACTGTGCTAACTTTCTTTTCCATAGTGGAAACACTTA70.4079254079254079No Hit
GAGTCTCACTCTGTCACCCAGGCTGGAATGCAGTGGCATGATCTCAGCTCACTGCAACTTCCATCTCCTGG70.4079254079254079No Hit
GCTCATATCTGTTCTTCAGCAGCCTGTCATAGGAACTGGATCCTACCTATGTTAATTACCTTATAGAACTA60.34965034965034963No Hit
TCTTTAGATTTTGGCAGCAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATAATGA60.34965034965034963No Hit
GTTTTCTGGAACATAATATATTTTGTTGACATTTCAAGGTGGTAAATGATCTCTGTTCAGATGAGATAAAA60.34965034965034963No Hit
GCAAACAATGTAAAACTTATGAACTGTTTATTTTTGGAATTTTCCATGTCATATTTTTGGTCTACAGGTAA60.34965034965034963No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC60.34965034965034963No Hit
TGTCTGTAGTTCCTGCTACTCGAGGCTGAGCTGGAGGATCACTTGAGCCCAGGAGGCAGAGGTTGCAGTGA60.34965034965034963No Hit
CCCACATCCTGGGTCACAGACTGGGATGAGCAACGGGCTGAAGGCACGTTTCCCGGGAAGATCTGAACTGG60.34965034965034963No Hit
GTATTATTAAAAACATATGGATCCCCATGAAGCCCTACTACACCAAAGTTTACCAGGAGATTTGGATAGGA50.2913752913752914No Hit
GTTCTCTTCATAACTGCCTTGACATTTTGGGTTGTTCTGTTCTGTTAATTTTCTTTTGCTTTTTTGTGTTT50.2913752913752914No Hit
CTCTGTCACCGCCATGCAACTCCATGCCTATTTACTGGAAACCTGTTATGCCAAACAGTTGTACCACTGCT50.2913752913752914No Hit
GATTTTGATCCCCTTGGAATATTAAGAGGTCAACACGTGATTGTTGTACGTACACATTGTGCTCTGGAGTG50.2913752913752914No Hit
GTCCTAGCGGCCCTTCTGGAGTCTCCCGTCACTCAGGACATCATCTGCGACGTGGGGATCATTTTTCTGGA50.2913752913752914No Hit
GTGGTGGAATCCCCTTTTCCCCATCTTCTTCTTTCAGATCATTAATGAGCAGAATAAAAAGAGTAAAATGG50.2913752913752914No Hit
GTGCTATCCTACGAAATCATTTGTTTCTAAGTTGTGTTTATTCCTGGAGTGACATGCCACCCCGAATGGCT50.2913752913752914No Hit
CTCCCCCACACCCTGGTGTTTTAAAATGAAGAAAAAAAACCATCACTTTTTATACAAAAACTCTTAATAAT50.2913752913752914No Hit
GGGGTCTCCTTGGCAGCTGTATTCTGGAGTCTGGATGTTGCTCTCTAAAGACCTTTAATAAAATTTTGTAC40.2331002331002331No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA40.2331002331002331No Hit
GCATCAGAAAAGTATAGTAAGAAGAAACTGAATTTGAAGTGGATTCTTACAAAGGAAAAAGAAAATCACTA40.2331002331002331No Hit
GGAGCTGATGCGATCAACTGGAAGAAAGGGTATCAGCAATGGAAGATGAAATGAATGAAATGAAGTGAGAA40.2331002331002331No Hit
GCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATGTATGCCAAGCGTGCCTTTGTTCA40.2331002331002331No Hit
TATGAAACAGCTGGGTCTTTGGCCATAAGAAGTAAAGATTTGAAGACAGAAGGAAGAAACTCAGGAGTAAG40.2331002331002331No Hit
GTCCTTGGACTGTTTTGAAAAGTTTCCTAGGATGTCATGTCTGCTTGTCAAAAGAAATAATCCCTGTAATA40.2331002331002331No Hit
GGGAGGCCGAGGCGGGCAGATCACGAGGTCAGGAGATCGAAGCCATCCTGGCTAACACGGTGAAACTCCGT40.2331002331002331No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAA40.2331002331002331No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17482517482517482No Hit
GGAATGCCACTTCAAGGAATATGCCCTAAGACACAAATTCAACAGGAAAAAAGAGCAAAGATGTTCAATTG30.17482517482517482No Hit
CCTGCAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACCTGAGGTCGGGAATTTGAGACCAGCCTG30.17482517482517482No Hit
CACCCCCTTCTGTGGGAAAGGAAACAGGTGCTGAGCCCAAGAGACTCTGGTACCCACTGCTGGCTCATGAA30.17482517482517482No Hit
CCCTCTCTCCCATTCTGTGATGAGTACAGCAGAGACCTTCCTGCTTTTTACTGGGGACTCCAGATTTTCCC30.17482517482517482No Hit
ACTTGACATGTTGGCATTCATATTAATTACGGATGTAATTCTGTGTATCTTACTGTGACTCCGAAAACTGT30.17482517482517482No Hit
TTCCCTACACGACGCGGGGCCTGCGCTGGGGACGGCTCTGGGGACTGCGGCCGGCGCCGGGACCTGGAGGG30.17482517482517482No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17482517482517482No Hit
GGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAAATATTTTCTGTTAATAAATTGCCTTCATGTA30.17482517482517482No Hit
GAGGTGGAGGTTGCAGTGAACCAAGATCATACTATTGCACTCCAGCCTGGACAACAAGAGCAAAACTTCGT30.17482517482517482No Hit
GTCCTTGGACTGTTTTGAAAAGTTTCCTAGGATGTCATGTCTGCTTGTCAAAAGAAATAATCCCTGTAATT30.17482517482517482No Hit
GAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAG30.17482517482517482No Hit
ACGCGGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17482517482517482No Hit
GCAGATTGCCTGAGGTCAGGAGTTCGAGACCAGCCTGGCTAACATGGTGAAACCCTGTCTCCATTAAAAAT30.17482517482517482No Hit
GTGAGTTAAGATCATGCCACTGCACTCCAGCCTGGAAGACACAGCAAGACTCCATCTCAAAAAAAAAAAAA30.17482517482517482No Hit
CCTCCATCCTTCTCCCAGGATGGTGAAGGGGGACCTGGTACCCAGTGATCCCCACCCCAGGATCCTAAATC30.17482517482517482No Hit
GGTAAGCACCAGGACGCCCTCGAGGTTGCTCTGGGATCCCCCCACAGCCCCTGGTCAGTCTGCCCTTGTCA30.17482517482517482No Hit
GCCACATTCTATTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17482517482517482No Hit
CTACTAAAAATTCAAAAAAAAAAATTAGCCGGGCGTGATGGTGGGCGCCTGTAGTCCCAGCTACTCGGGAG20.11655011655011654No Hit
GGTCAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGAGAAGATTAAGAGTGTTGGGGGGGCCTGTGTCCTGGTGGCTTGAAGCCACATGGAGGGAGTTTCATTAA20.11655011655011654No Hit
ACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
CTTCTTTGATAAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
TTGCTATAGAGCCTACCTGTATGTCAATAAACAACAGCTGAAGCACCAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGGAGAGCAGGGTTGGGGGAGAGGCTGAGGAGAGTGTGACATGTGGGGAGAGGACCAGCTGGGTGCTTGGG20.11655011655011654No Hit
CTGTTGACTTGTAAAATCAATAAAGTCTTAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
TCCCTACACGACGCGGGAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGTCTGAATATTAAAGCTCTTCATTTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
TGTTTTGCATGTATGACTTAATAAATCCTTGAATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
CCTCTCCCTTCTGTGGGTCGCTCCCAGAGCCATGGCCCATGGGAAGGACAGAGTGTGTGTGTCCTTGGGGC20.11655011655011654No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA20.11655011655011654No Hit
GGTAGCTTCTATGTAATTCGCAATGATTCCATCTAAATAAAAATTCTATAATCTACAAAAAAAAAAAAAAA20.11655011655011654No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAAACAACTTTCTTAACTATCCTAACAAACCTT20.11655011655011654No Hit
GGTCTGAATATTAAAGCTCTTCATTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGTCTGAATATTAAAGCTCTTCATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCATCCTAAAATTAATTCCCCTAAAAATCT20.11655011655011654No Hit
GGGTGATGACCTGCTTCCTGCTGGCACTGAGGATTATATCCATATAAGAATTCAACAGAGAAACGGCAGGA20.11655011655011654No Hit
GGATGGATGCAAAGTATAAATTTGTGTAATATTACTTTAACATGCCCATATTTTACTTGGAAATATTAAAA20.11655011655011654No Hit
GGCAGGAGAATCGCTTGAACCCAAGAGGCGGAGGTTGCAGTGAGCCAAGATCGCACCACTGCACTCAAGCA20.11655011655011654No Hit
GCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTGGAAAAAAAAAAAA20.11655011655011654No Hit
CCTCCAGCTGTACTAGTGCCATTGGAATAATAAATTTGATAAGATAATAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
TGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATTTGTCA20.11655011655011654No Hit
GATCTAAGAGAATGCTGGCTATTAAAAGATGCAAGCATTTTGAACTGGGAGGAGATAAGAAGAGAAAGGGC20.11655011655011654No Hit
ATCGCGCCACTGCACTCCAGCCTGGGTGACATAGCAAGACTCTATCTCAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GTATATATTCATCTGAGATAAAAATATAAAAAGAATTATAAACCCTAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GTTGAGTTTTCTGTACATAAAAATAATTGAAATAATACAAATTTTCCTTCAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GACTGCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCCGATCACC20.11655011655011654No Hit
GAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGCTCGAACGAGTGGAAGACGGTTGTGGGCGGTGCCATGTTCTTCATCGGTTTCACCGCGCTCGTTATCAT20.11655011655011654No Hit
TGTTTGTTGGGACAGAAATAACCCAGAGCCCTGGAACAAACTGGGTCCCAATGATCAATACAAGTTCTACT20.11655011655011654No Hit
GGTTTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GTGTGTGAAAACAATATTGTATACTACCATAGTGAGCCATGATTTTCTAAAAAAAAAAATAAATGTTTTGG20.11655011655011654No Hit
ACCTCTCCCTGCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
CCTATTACCTCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA20.11655011655011654No Hit
ACTCCAGAACAGTACCAGATCCTGAAGGAAAATTACGGGCAGAAGGAGGCTGAGAAAGTGGCCCGGGTGAA20.11655011655011654No Hit
CAGATGGAGCAGCTCAACGGGATCCTGCTGCACCTTGAGTCAGAGCTGGCACAGACCCGGGCAGAGGGACC20.11655011655011654No Hit
CAGTACCTGCTCTCAAATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGTAGCTTCTATGTAATTCGCAATGATTCCATCTAAATAAAAGTTCTATGATCTGCAAAAAAAAAAAAAAA20.11655011655011654No Hit
GCCCGAATCCGGGACACACTTCGGCGAGTGCTTAACCTACCTCCCAACACCATTATGGAATACAAAACTCA20.11655011655011654No Hit
AGACTGAACCGAATAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGTGGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTGCTTAGCAAGTTCCGGATAGC20.11655011655011654No Hit
ATATTTTACATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GCGATTCCGAGGGTGACTGAGGCTACAGCTTCTATCACATGCCGAACTTTCTTGTGACAAATTGTCTGGAT20.11655011655011654No Hit
GGAGCTAAGTGCCTTTTTGTGTGCAACCACTTACCCTTTCTCTAAAAAACCTATTCTCAAAAAAAATCTAA20.11655011655011654No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GTTAAACACAACAAAGAATTTCTCTTGTCAATGGCCAACAGAGGGAAGGATACAAATGGTTCACAGTTCTT20.11655011655011654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)