FastQCFastQC Report
Wed 8 May 2024
EGAF00002876031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266288
Total Bases18.9 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29761.1175869735023733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8740.32821606681487714No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA7060.26512647960103347No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6410.24071681788139157No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.23320615273688639No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC6060.22757315387850746No Hit
GTATTGAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT5990.22494442107793064Illumina Single End PCR Primer 1 (100% over 39bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT5820.21856035570510124No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT5540.20804542450279395No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5270.19790602655771197No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA4830.1813825632398005No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG4380.16448356666466382No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA4070.15284203569068078No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA3830.14382923751727453No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG3620.13594303911554406No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.1355675058583188No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG3550.13331430631496724No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3430.12880790722826413No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA3420.12843237397103888No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA3410.1280568407138136No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC3370.12655470768491256No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC3270.12279937511265998No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA3180.11941957579763264No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA3130.11754190951150634No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA3120.11716637625428108No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTG3110.11679084299705582No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.1160397764826053No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2980.11190891065312744No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2920.10965571110977587No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT2890.10852911133810009No Hit
CTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGT2890.10852911133810009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)