FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131
Sequences flagged as poor quality0
Sequence length71
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48822.90004692632567No Hit
CTCAAGGACAAGATGCGCAAAGCGGTGGCTGAGGAGCTGGCCAAGTGACTTGTGCCAGCAGCATGGACTGC532.4870952604411074No Hit
ATCCTTGCTTCCCTGAGCGTTGGCCCGGGAGGAAAGAAGATGGTGCTGGATCTGGATTTGTTTCGGGTGGA512.3932426091037073No Hit
TATCTATGAGGAGACTCGAGGTGTGCTTAAGGTTTTCTTAGAGAACGTTATTCGAGACGCCGTCACCTATA442.0647583294228062No Hit
CCTATACGGAGCACGCCAAGCGCAAAACTGTCACAGCCATGGATGTAGTATATGCCCTGAAACGTCAGGGG401.8770530267480057No Hit
GGCTTCTGCCCTCTGCAGCTGGGACCCCTCACTTGCCTGCCATGCTCTGCTCGGCTTCAGTCTCCAGGAGA341.5954950727358048No Hit
GGATAAAGGAGGGGACCCAGCCCTCATCCGAGAGACGCAGGAGAAGCGCTTCAAGGACCCGGGACTAGTGG341.5954950727358048No Hit
AGGGGAATGTGTTCAAAAACAAGCGGATTCTCATGGAACACATCCACAAGCTGAAGGCAGACAAGGCCCGC341.5954950727358048No Hit
CCTACATCTTGAAAGATAATTTCTCTGAGTCTACAATTCCCAGCTGACAATGTTTATCTTCCAGTACTTTA311.4547160957297043No Hit
GGCATGATACATAGTAAAATAATGTTCCAGGTTAACCAGGAAAGAAGCAAGTGCGACTGTGCCTTTGGTTA281.313937118723604No Hit
AGATTCACCGTCCTAGAGCTTAGGCCTGTCTTCCACCCCTCCTGACCCGTATAGTGTGCCACAGGACCTGG251.1731581417175034No Hit
GAGTTATATAATGAGATTTCTAGAAAGCTCTGGACATGGGTACGATGTGTTTTGCTTCTCTGTATAATGTC231.0793054903801031No Hit
CAGCCATGGATGTAGTATATGCCCTGAAACGTCAGGGGCGCACTCTGTATGGCTTCGGCGGCTGAATCTAA231.0793054903801031No Hit
GTTTGGTATTTTGTCTTATTGTTTCTCTACTAAGAACTCTTTCTTAAATGTGGTTTGTTACTGTAGCACTT231.0793054903801031No Hit
CTTCCCATTTCTGATTGTTGTATAGCTTTCGATTTTGCTTACAGTAGTTCCCCCTTATCTTCGGGAGATAC221.0323791647114031No Hit
TGATTCATGAGCCAGAACCACATATTCTTCTCTTTAGACGACCTCTTCCAAAAGATCAACAAAAATGAAGT221.0323791647114031No Hit
CATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.9854528390427029No Hit
GATCCAGGGGATGGAGTGCAATGGCGCGATCTCAGCTCACTGCAACCTCCACCTCCAGGATTCAAGCAATT210.9854528390427029No Hit
GCAGCAGAAACATCAGTATCAGCAGCGTCGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCC200.9385265133740028No Hit
GGATTACAGGTATGAGCCACAACGCCCAGCCATCATTTATCTTTTTCACTCATTTTTTCATGAGTATGCAG190.8916001877053027No Hit
GGCTCTGAGCTGCACTGGCCCCGGTGCTGGCATCTGGTGGAGCGGACCCACTCCCCTCACATTCCACAGGC190.8916001877053027No Hit
ATCTAATCAAGGGAGTATTTTCTGCGTAAAAGCCCTTTGTCAGATAGTTGACAATCTAAGGCATTATCTTT170.7977475363679024No Hit
AGGAGATATAACTAATGCTAAATGAGGAGTTAATGGGTGCAGCACACCAGCATGGCAGATGTATATATATG170.7977475363679024No Hit
GCCTGTACCTGAAGGTGAAGGGGAATGTGTTCAAAAACAAGCGGATTCTCATGGAACACATCCACAAGCTG160.7508212106992023No Hit
GTATCCAGGTTCCCTCCCAGAGGAGCCACCAGTTCTCATGGGTGGCACTCAGTCTCTCTTCTCTCCAGCTG160.7508212106992023No Hit
GGACCTGGACAGTGAAGGGAGCCCGGGCAGCCACCGTCTCCAGAGCCCTGGGCAGCATTTTCCAGCAAGAT160.7508212106992023No Hit
CCCAGAATTTCAGCTTCAGCTTAACTGACAGACGTTAAAGCTTTCTGGTTAGATTGTTTTCACTTGGTGAT150.7038948850305021No Hit
GCTCCTGGCTGGCCAGGCTGAGGCCCGCAGGTCTAAGACCAAGGAAGCACGCAAGCGCCGTGAAGAGCGCC150.7038948850305021No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA140.656968559361802No Hit
GGAAAAGAAACCAAGAACTGAATTACTGTCTGCCCATTCACATCCCATTCCTGCCTTTTGCAATCATGAAA140.656968559361802No Hit
TCCCTACACGACGCGGGGAAAAAAAAATAAAAATCTGACTTAGTTTTAAAAAAAAAAAAAAAAAAAAAAAA130.6100422336931018No Hit
AAGCAGATCGAGAGCAAGACTGCTTTTCAGGAAGCCTTGGACGCTGCAGGTGATAAACTTGTAGTAGTTGA130.6100422336931018No Hit
GTCACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGTTAAAAAAAAAAAAAAAA120.5631159080244017No Hit
GTAGTATATGCCCTGAAACGTCAGGGGCGCACTCTGTATGGCTTCGGCGGCTGAATCTAAGAATACGCGGT120.5631159080244017No Hit
CTTATAATCTTAAATGTATTTTCCTTTGTTTAAGCTGCTGCTTCCTCTGTTTCATTGGATTGTGCCAGTTA120.5631159080244017No Hit
ATAGGAATCATGTCTGGTCGCGGCAAAGGCGGAAAAGGCTTGGGGAAGGGTGGTGCTAAGCGCCATCGTAA120.5631159080244017No Hit
TCAAGGACCCGGGACTAGTGGACCAGCTGGTGAAGGCAGACAGCGAGTGGCGACGATGTAGATTTCGGGCA120.5631159080244017No Hit
GGCAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCT110.5161895823557016No Hit
CACTCTGTACAAGAGCCAGGTGAGAATTTCAAGGATTATCGACTTCATATTGCACATTAAAGTTACAAATT100.4692632566870014No Hit
GTATATGCCCTGAAACGTCAGGGGCGCACTCTGTATGGCTTCGGCGGCTGAATCTAAGAATACGCGGTCTC100.4692632566870014No Hit
CCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGCTTAACTGACAGACGTTAAAGCTTT90.4223369310183012No Hit
TTCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCTGTAATATTTTTCCATCATAT80.37541060534960113No Hit
CACTTTGTCTGTACATACTGGCCTCTGTGATTACATAGATCAGCCATTAAAATAAAACAAGCCTCAAAAAA80.37541060534960113No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCA80.37541060534960113No Hit
GTACAAGAGCCAGGTGAGAATTTCAAGGATTATCGACTTCATATTGCACATTAAAGTTACAAATTAAAGTG80.37541060534960113No Hit
ATCAGACAGAATAATATTTTCTAGTTATTATGTGTAAGATGAGTTGCTATTTTTCTGATGCTCATTCTGAT70.328484279680901No Hit
GTGTAGACTCAGCCTCACTCTGTACAAGAGCCAGGTGAGAATTTCAAGGATTATCGACTTCATATTGCACA70.328484279680901No Hit
CCCTTTTTGAGCAAAATGAAGATGTTTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.328484279680901No Hit
GCTTAATTGCCTTTACAAGTACTTATGTACAACTTTGTATCAAATTTTCTGTAATAGTTTATGCTTTAGTA70.328484279680901No Hit
CTTATACAAATATTCAGAAGTGTAATAAAAATGTGAATGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.28155795401220085No Hit
GGCTTGAAGTCCCCCTCGCGAGTGCCTTCTTGCAATTACCTGCCTTAGCGGGTGTTGCGGGTCCCTCCTTC60.28155795401220085No Hit
GGACATCTACACCGAAAACCCATCTGTACATCACCATCATCAAAGACCAAAAGTAGATAAAACCACAAAGA60.28155795401220085No Hit
GGCTTCTGCCCTCTGCAGCTGGGACCCCTCACTTGCCTGCCATGCTCTGCTCGGCTTCAGTCTCCAGGAGC50.2346316283435007No Hit
CTAATTATCCATTCCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGCTTAACTGACAA50.2346316283435007No Hit
CATTAAAACATTTTTGATTACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2346316283435007No Hit
GTGCCGCAGTTTGTAGTGTTTTATCTTATCTACACATGGGGGACTGAAGAGTTCGAGAGATCCAAGAGGAA50.2346316283435007No Hit
CAAGGGAGGAGACTGCACCCCACTGCCTCTGGGCCCTGGCTGTGGGCAGAGGCCACCGTGTGTGTCCCGAG50.2346316283435007No Hit
TATACATCCTGTATGTGGTTCTCTCAAGTTTCTGGATTGCAGTAATGTATTTTGTTTAAATAAAACAAAAT50.2346316283435007No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2346316283435007No Hit
TACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAAAAAAAAAAAAAAAAAAAAAA50.2346316283435007No Hit
GTGTAAAGCATTCCAACAAAGGGTTTTAATGTAGATTTTTTTTTTGCACCCCATGCTGTTGATTGCTAAAT40.18770530267480057No Hit
GGCGTCTTTTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18770530267480057No Hit
ATACAGAGTCAATATATTTTTTCAGAGAAAGTTATTCGCTCGATTTTTTCTGAATCATAATTAAACTTTAT40.18770530267480057No Hit
CTAATTATCCATTCCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGCTTAACTGACAG40.18770530267480057No Hit
CTAAATAACAATTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18770530267480057No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACGTTCA40.18770530267480057No Hit
GGGCTTCCCCAAGGATTTATTGACATTGCTACTTGAGTGTGAACAGTTACCTGGAAATACTGATGATAACA40.18770530267480057No Hit
CTGTGACTGCCTGGATGAAGACTGCTTAGGATGTTTCTATGCTTGTCCTGCCTGTGGTTCTACCAAGTGTG40.18770530267480057No Hit
GTTTTTAGACCTGTGTTACTAAAAAAAAGATGAATGTCCTGAAAAGGGTGTTGGGAGGGTGGTTCAACAAA30.14077897700610043No Hit
GGTGTCTTATAATCTTTTACCTATTTCTTACAATTGCAAGATGACTGGCTTTACTATTTGAAAACTGGTTT30.14077897700610043No Hit
ACTATGTGCAGTCCATGGATGTGGCTGCTTTCAACAAGATCTAAAATCCATCCTGGATCATGGCATTTAAA30.14077897700610043No Hit
GACTTGAGACCAGTTGAATAAAAGTGCACACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14077897700610043No Hit
TGTGTATACTCATGATAGGTCAGAAAGTGTATCCGCTACAATAAAGTTCTGGTTCTAACTAAAAAAAAAAA30.14077897700610043No Hit
GTCTAATCTGAATTTGCATTCCATGGTGTTAACATGGTATATGTATTGTTATTAAAGTAAGTGACCCATGT30.14077897700610043No Hit
TATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGA30.14077897700610043No Hit
GAGTTAATAAATGCCTTGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14077897700610043No Hit
GTACAGAGAGCTGTGTGCATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAA30.14077897700610043No Hit
CTGTACTACAATCAACTTTATTTTGAAATCATGTAAAATAAGATATTAGACTGTTTTTTGAATAAAATATT30.14077897700610043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)